不同分类位置布鲁氏菌菌株中 omp25 和 omp2a 基因的变异性

N. A. Osina, E. Boolgakova, A. G. Osin, I. G. Shvidenko, S. A. Shcherbakova
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摘要

研究的目的是确定布鲁氏菌omb25和omb2a基因的结构差异,从而确定它们的分类位置。研究对象是 GenBank NCBI 数据库中 48 个布鲁氏菌菌株完整基因组的核苷酸序列。为了评估序列同源性,使用了 BLAST 算法和 MEGA 11 程序。在 13 种布鲁氏菌和布鲁氏菌属未知种的附属物中,HindIII、EcoRV 和 EcoRI、AluI 对基因组区域的限制性图谱进行了模拟再现,包括 omp25 和 omp2a 基因,两侧分别使用引物 25A-25B 和 2AB-2AA。在 omp25 基因中,检测到 11 个非同义突变和 24 个同义突变;两个缺失:∆103-108 bp - 在 В. nosferati 中,∆562-597 bp - 在 В.Ovis;В. vulpis 的 ACT 插入在 585 bp 之后。在所研究的布鲁氏菌菌株中,omb2a 基因的变异性明显更高。在В. abortus 1、2、4 生物变种中发现了 138 个 SNPs,其中 60 个导致氨基酸替换,2 个形成终止密码子,还有一个缺失 ∆424-561 bp。omp2a 和 omp25 基因的单个突变既有群体特异性--针对多个物种,也有独特性--针对病原体的特定物种或生物变种。omp25 和 omp2a 基因的等位基因图谱比所研究的限制性图谱具有更高的分辨率。已确定的 omp25 和 omp2a 基因结构的变化与布鲁氏菌的个别种类和生物变种在某些带菌者机体内的循环有关。这些基因显示出缺失、插入和单个多态核苷酸的存在,这些多态核苷酸是病原体的物种、物种群和某些生物变种所特有的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Variability of omp25 and omp2a Genes in Brucella Strains of Different Taxonomic Position
The aim of the study was to identify structural differences in the Brucella omp25 and omp2a genes, which make it possible to determine their taxonomic position.Materials and methods. The objects of the study were the nucleotide sequences of the complete genomes of 48 Brucella strains presented in the GenBank NCBI database. To assess sequence homology, the BLAST algorithm and the MEGA 11 program were used.Results and discussion. In 13 species of Brucella and Brucella spp. with unknown species appurtenance, HindIII, EcoRV and EcoRI, AluI restriction profiles of genome regions have been reproduced in silico, including, omp25 and omp2a genes, flanked by primers 25A-25B and 2AB-2AA, respectively. In the omp25 gene, 11 non-synonymous and 24 synonymous mutations have been detected; two deletions: ∆103–108 bp – in В. nosferati, ∆562–597 bp – in В. Ovis; and ACT insertion after 585 bp – in В. vulpis. The variability of the omp2a gene in the studied Brucella strains was significantly higher. 138 SNPs have been identified, of which 60 lead to amino acid substitutions, 2 – form stop codons, and an additional deletion ∆424–561 bp – in В. abortus 1, 2, 4 biovars. Single mutations in the omp2a and omp25 genes had both group specificity – for several species, and unique – for a specific species or biovar of the pathogen. Allelic profiles of the omp25 and omp2a genes have greater resolution than their restriction profiles studied. The identified changes in the structure of the omp25 and omp2a genes correlate with the circulation of individual Brucella species and biovars in the organism of certain carriers. These genes show the presence of deletions, insertions and single polymorphic nucleotides specific to species, groups of species and, in some cases, biovars of the pathogen.
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