用 Wrightia tinctoria 果实提取物合成的银纳米粒子的抗菌活性

Arpana Ashokrao Durge, Utpal Dongre, Utkarsh Ravindra Moon
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引用次数: 0

摘要

纳米粒子是通过各种化学方法合成的,产量较高,但并不十分环保,而且会对活细胞造成危害。这可能是由于在合成过程中过度使用了有害化学物质。纳米粒子合成的绿色方法被认为是无毒、无害和环保的,因此受到全世界的广泛关注。除此之外,纳米粒子还可应用于各个科技领域。利用各种植物化学物质合成的纳米粒子也能有效地抑制各种微生物种群。本研究旨在从 Wrightia tinctoria 的果实提取物中合成银纳米粒子(AgNPs),并评估其对革兰氏阳性和阴性细菌菌株的抗菌能力。使用不同的溶剂萃取 Wrightia tinctoria 豆荚合成了银纳米粒子。通过检测溶液颜色的变化来观察银纳米粒子的形成。通过紫外可见光谱和 400 纳米至 800 纳米的光谱监测来检测纳米粒子的存在。在 425 纳米波长处出现的一个小峰值表明存在纳米银粒子。在研究的后一部分,监测了绿色合成银纳米粒子对大肠杆菌和金黄色葡萄球菌生长的抑制作用。抑制区的出现进一步证实了合成纳米粒子的抗菌效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial activity of silver nanoparticles synthesized from Wrightia tinctoria fruit extracts
Nanoparticles are synthesized by using various chemical methods in higher yields but they are not very environmentally friendly and have hazardous effects on living cells. This can be attributed to the overuse of hazardous chemicals involved in the process. The green approach of nanoparticle synthesis is widely gaining attention worldwide as it is considered as nontoxic, harmless and ecofriendly. Apart from this they also have multiple applications in various fields of science and technology. Nanoparticles synthesized by using various phytochemicals are also effective against a variety of microbial populations. The objective of this study is to synthesize silver nanoparticles (AgNPs) from the fruit extracts of Wrightia tinctoria and evaluating its antimicrobial capacities against gram-positive and negative bacterial strains. Silver nanoparticles were synthesized using different solvent extracts of Wrightia tinctoria pods. The formation of silver nanoparticles was noted by detecting the change in color of the solution. The presence of nanoparticles was detected by performing UV visible spectroscopy and monitoring the spectrum from 400 nm to 800 nm. A small peak at 425 nm suggested the presence of silver nanoparticles. In a later part of the study, the inhibitory effect of green synthesized silver nanoparticles on the growth of E. coli and S. aureus was monitored. The appearance of a zone of inhibition further confirmed the antimicrobial effect of the synthesized nanoparticles.
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