辣椒(辣椒属)炭疽果腐病抗性筛选方法的评估

IF 0.2 Q4 HORTICULTURE
B. Vanlalneihi, Radha P L, Subbaraman Sriram, Kambham Madhavi Reddy
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引用次数: 0

摘要

由 Colletotrichum spp.引起的炭疽病果实腐烂是一种严重的生产制约因素,会造成辣椒严重的市场产量损失。对辣椒品种对 Colletotrichum spp.的抗性进行田间评估取决于影响病害表现的各种因素,如土壤条件、温度、降雨量、湿度和其他难以控制的变量,因此准确性较低。此外,不同 Colletotrichum 种类交叉感染的几率很高,导致对特定病原体种类和分离物的检测无法得出结论。为了确定一种稳定可靠的筛选方法,使用特定的病原体分离物对不同的辣椒品种进行了体外针刺法和无伤口喷雾法试验。在针对球孢子菌分离物 "IHRCg-1 "筛选辣椒品种时,除 PBC80 外,体外针刺法与无伤口喷雾法呈现出正相关。生物测定方法的改变影响了品种 PBC 80 的病害反应模式,可能是针刺破坏了基部角质层的防御机制,而喷雾接种法保留了这一机制,表明抗性模式各不相同。然而,在 PBC 81 中,两种接种方法都能观察到其对两种分离物(即 C. truncatum 'IIHR Ct-1' 和 C. gloeosporioides 'IIHR Cg-1')的稳定抗性模式,而在 PBC 80 中,两种接种方法都能观察到其对 C. truncatum 'IIHR Ct-1'的稳定抗性模式,这说明在两种接种方法中都有抗性基因的表达。根据病害发展模式,红熟辣椒对 C. truncatum 和 C. gloeosporioides 的感染表现出不同的反应。10 DAI 和 14 DAI 是炭疽病感染的高峰期,可分别记录辣椒成熟果实上 "IIHR Cg-1 "和 "IIHR Ct-1 "的感染情况,以评估抗性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of screening methods for anthracnose fruit rot resistance in chilli (Capsicum spp.)
Anthracnose fruit rot caused by Colletotrichum spp. is a serious production constraint causing severe marketable yield loss in chilli. Field evaluation of chilli accessions for resistance to Colletotrichum spp. depends on various factors affecting disease expression such as edaphic conditions, temperature, rainfall, humidity and other variables that are difficult to control, therefore considered less accurate. Also, high chances of cross contamination with different Colletotrichum species leads to inconclusive assays for specific pathogen species and isolate. To identify a stable and reliable screening method, various chilli accessions were subjected to in vitro pin-prick and non-wounding spray methods using a specific pathogen isolates. When chilli accessions were screened against C. gloeosporioides isolate ‘IHRCg-1’, the in vitro pin- prick method showed positive correlation with the non- wounding spray method, except in the accession PBC80. The change in bioassay influenced the disease reaction pattern in the accession PBC 80, probably the pin pricks break the basal cuticle defense mechanism that was retained in spray inoculation method indicating varied resistance pattern. However, in the accession PBC 81 stable resistance pattern was observed against isolates of both species viz., C. truncatum ‘IIHR Ct-1’ and C. gloeosporioides ‘IIHR Cg-1’ and in the accession PBC 80 against C. truncatum ‘IIHR Ct-1’ in both the inoculation methods that depicted the expression of resistance genes during both methods of inoculation. Based on disease development pattern, the red ripe chilli expressed a variant reaction to infection by C. truncatum and C. gloeosporioides. The peak anthracnose infection at 10 DAI and 14 DAI is an accurate duration to record ‘IIHR Cg-1’ and ‘IIHR Ct-1’ infection, respectively on chilli ripe fruit for assaying the resistance.
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来源期刊
Journal of Horticultural Sciences
Journal of Horticultural Sciences Agricultural and Biological Sciences-Plant Science
CiteScore
0.30
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0.00%
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审稿时长
6 weeks
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