Influence of Seaweed Extracts on The Antioxidant System and Activity in Spinacia oleracea as Edible leafy Vegetable Plant

The current study sought to determine the effects of Colpomenia sinuosa and Sargassum linifolium aqueous extracts on non-enzymatic and enzymatic antioxidants in Spinacia oleracea L. leaf extract. Glutathione synthase (GSS, EC: 6.3.2.3) and -glutamyl cysteine synthetase (-GCS, EC: 6.3.2.2) are involved in the glutathione biosynthesis process. Treatment of S. oleracea with seaweed extracts increased the level of reduced glutathione (GSH), oxidized glutathione (GSSG), and total glutathione at lower concentrations. Total phenols and total flavonoids in S. oleracea leaves accumulated more rapidly after treatment with seaweed extracts. The activity of the enzyme’s phenylalanine ammonia lyase (PAL, EC: 4.3.1.25), chalcone synthase (CHS, EC: 2.3. 1.74), and chalcone isomerase (CHI, EC: 5.5.1.6) involved in the production of phenylpropanoid and flavonoid in S. oleracea leaves rose in a dose-dependent manner. Glutathione reductase (GR, EC 1.6.4.2), glutathione peroxidase (GPX, EC: 1.11.1.9), and glutathione-S-transferase