利用联用液相色谱法（UPLC-MS/MS）同时测定头孢吡肟和他唑巴坦

Q3 Pharmacology, Toxicology and Pharmaceutics

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI:10.52711/0974-360x.2024.00011

Jabeen Jabeen, Suma B.V.

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引用次数: 0

摘要

本研究采用 UPLC-MS/MS 方法，建立并验证了同时测定头孢吡肟和他唑巴坦这两种药物的联用分析方法。采用 C18 BEH 分析柱。流动相为 0.2% 甲酸水溶液和乙腈，体积比为 20:80。样品采用+ESI电离，在MRM模式下进行检测。头孢吡肟和他唑巴坦的保留时间分别为 0.80 分钟和 0.96 分钟。头孢吡肟和他唑巴坦在研究范围内呈线性反应，头孢吡肟的 r2 = 0.9955，他唑巴坦的 r2 = 0.9816，精密度和准确度均有提高。头孢吡肟和他唑巴坦的最低检测限分别为 66ng/ml 和 8.3ng/ml。头孢吡肟和他唑巴坦的 LOQ 分别为 200ng/ml 和 25ng/ml。根据研究结果，我们按照 ICH 法规开发并验证了同时测定头孢吡肟和他唑巴坦的 UPLC-MS/MS 联用方法。

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Simultaneous determination of Cefepime and Tazobactam by using Hyphenated Liquid Chromatography (UPLC-MS/MS)

The present study deals with establishing a hyphenated analytical method and validating the same for the determination of the drug combination of Cefepime and Tazobactam simultaneously by UPLC-MS/MS. A C18 BEH analytical column was employed. A mobile phase of 0.2 percent formic acid in water and acetonitrile were used in a 20:80 v/v ratio. Samples are ionized using +ESI ionization and detected in MRM mode. The retention time of 0.80 min and 0.96 min were observed for Cefepime and Tazobactam respectively. The drugs have linear responses in the investigated range, with r2 = 0.9955 for Cefepime and r2 = 0.9816 for Tazobactam with increased precision and accuracy. The LOD for Cefepime and Tazobactam was 66ng/ml and 8.3ng/ml respectively. The LOQ for Cefepime and Tazobactam was 200ng/ml and 25ng/ml respectively. According to the findings, the hyphenated UPLC-MS/MS method was developed and validated as per ICH regulations to determine Cefepime and Tazobactam simultaneously.

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来源期刊

Research Journal of Pharmacy and Technology Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal, devoted to pharmaceutical sciences. The aim of RJPT is to increase the impact of pharmaceutical research both in academia and industry, with strong emphasis on quality and originality. RJPT publishes Original Research Articles, Short Communications, Review Articles in all areas of pharmaceutical sciences from the discovery of a drug up to clinical evaluation. Topics covered are: Pharmaceutics and Pharmacokinetics; Pharmaceutical chemistry including medicinal and analytical chemistry; Pharmacognosy including herbal products standardization and Phytochemistry; Pharmacology: Allied sciences including drug regulatory affairs, Pharmaceutical Marketing, Pharmaceutical Microbiology, Pharmaceutical biochemistry, Pharmaceutical Education and Hospital Pharmacy.