在丝裂霉素 C 诱导的氧化应激条件下,λ干扰素对小鼠体内活性氧生成的影响

G. Vostroilova, N. Khokhlova, D. I. Shabanov, A. Korchagina, D. D. Morozova, A. V. Nekrasov
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摘要

本研究旨在研究在丝裂霉素琥珀酸诱导的氧化应激条件下,物种特异性重组牛λ干扰素(IFN-λ)对小鼠肝脏和骨髓细胞中活性氧(ROS)生成的影响。实验对象为雌性白色实验鼠。实验共分为四组,每组 6 只小鼠:阴性对照组(I 组);注射三倍剂量 IFN-λ(0.1 毫升/千克)的小鼠组(II 组);除注射 IFN-λ 外,还注射可诱导自由基氧化过程的细胞毒性药物--丝裂霉素 C(剂量为 10 毫克/千克)的小鼠组(III 组);以及仅注射丝裂霉素 C 的小鼠组(IV 组)。我们通过 2',7'-二氯二氢荧光素二乙酸酯氧化形式的荧光强度,研究了小鼠肝脏细胞悬浮液的浓度和存活率,以及动物肝脏和骨髓细胞中细胞内 ROS 的相对含量。健康小鼠肝悬液中细胞的浓度和存活率并没有随着 IFN-λ(第二组)的引入而发生变化,这表明 IFN-λ 对这些细胞没有毒性作用。给 II 组小鼠注射 IFN-λ 后,研究细胞中的 ROS 水平升高(肝细胞中的 ROS 水平升高 1.3 倍,骨髓细胞中的 ROS 水平升高 2.9 倍)。在丝裂霉素 C 诱导的氧化应激条件下,小鼠体内的 ROS 水平有所下降(与 IV 组小鼠相比,III 组动物肝细胞和骨髓细胞中的细胞内 ROS 含量分别减少了 1.9 倍和 7.2 倍)。这些变化可能表明体内 IFN-λ 氧化还原平衡趋于正常,也可能与 IFN-λ 的免疫调节活性有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of interferon lambda on the generation of active oxygen species in mice under conditions of oxidative stress induced by Mitomycin C
This research was aimed at studying the effect of species-specific recombinant bovine interferon lambda (IFN-λ) on the generation of reactive oxygen species (ROS) in mouse liver and bone marrow cells under conditions of mitomycin Cinduced oxidative stress. The experiment included female white laboratory mice. There were formed four groups of 6 animals each: the negative control group (group I); the group of mice that received a three-fold injection of IFN-λ at a dose of 0.1 ml/kg (group II) and mice that, in addition to IFN-λ, were administered a cytotoxic drug that induced free radical oxidation processes - mitomycin C at a dose of 10 mg/kg (group III), as well as the animals receiving only mitomycin C (group IV). We studied the concentration and viability of a cell suspension obtained from the liver of mice, as well as the relative content of intracellular ROS in the liver and bone marrow cells of animals, assessed by the fluorescence intensity of the oxidized form of 2',7'-dichlorodihydrofluorescein diacetate. The concentration and viability of cells in the liver suspension of healthy mice did not change with the introduction of IFN-λ (group II), indicating the absence of a toxic effect of IFN-λ on these cells. An increase in the level of ROS in the studied cells was detected when IFN-λ was administered to mice of group II (an increase in the level of ROS by 1.3 times in liver cells and by 2.9 times in bone marrow cells, relative to the mice of group I) and a decrease in the level of ROS in the mice under conditions of oxidative stress induced by mitomycin C (reduction in the content of intracellular ROS by 1.9 and 7.2 times in liver and bone marrow cells in the animals of group III, relative to the mice of group IV). The presented changes may indicate the normalization of IFN-λ redox balance in the body and, probably, appear in connection with the immunomodulatory activity of IFN-λ.
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