从北方邦甘蔗根瘤土壤中分离的细菌的生化特征

IF 0.2 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
N.S. Yadav, Sakshi Issar, R.K. Gaur
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引用次数: 0

摘要

此外,这些土壤样本被用于分离和鉴定细菌,以确定其最大的 PGPR 特性和生化潜力。从土壤样本中总共收集了 10 株细菌分离物,在营养琼脂平板中进行亚培养,并通过生化测试进行鉴定。其中一株细菌呈阴性,其余 9 株细菌的革兰氏染色均呈阳性。所有分离出的细菌在柠檬酸盐测试中均呈阳性。3 株分离菌株在 MR 试验中呈阳性,2 株在 VP 试验中呈阳性。5 个分离菌株在明胶酶测试中呈阳性,9 个分离菌株在硝酸还原酶测试中呈阳性。2 个分离菌株在淀粉水解试验中呈阳性,7 个分离菌株在磷酸盐溶解试验中呈阳性,3 个分离菌株在嗜苷酸盐产生试验中呈阳性。这些结果表明,从土壤样本中分离出的细菌有氮化杆菌属(Azotobacter Species)、氮青霉属(Azospirillum Species)、假单胞菌属(Pseudomonas species)、芽孢杆菌属(Bacillus Species)、根瘤菌属(Rhizobium Species)、沙雷氏菌属(Serratia Species)和大肠杆菌(E. coli)。这些细菌菌株具有最大的 PGPR 特性。经过适当验证后,这些细菌菌株可用于制造生物肥料,通过提高土壤肥力来提高作物产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biochemical characterization of bacteria isolated from Rhizosphere soils of sugarcane grown in Uttar Pradesh
Rhizospheric soil samples of Sugarcane fields were collected from distinct regions of U.P. Further, these soil samples were used for the isolation and characterization of bacteria to identify their maximum PGPR traits and biochemical potential. A total of ten bacterial isolates were collected from the soil samples and sub-cultured in the nutrient agar plates and characterized through Biochemical test. One bacterial strain was found negative and the other 9 isolates were found positive for gram staining. All bacterial isolates were positive for citrate test. 3 isolates were found MR positive and 2 positives for VP test. 5 isolates were positive for gelatinase test and 9 isolates were positive for nitrate reductase test. 2 isolates were positive for starch hydrolysis test, while 7 isolates were found positive for phosphate solubilization test and 3 isolates were positive for siderophore production test. These results showed that the bacterial isolates from soil sample were Azotobacter Species, Azospirillum Species, Pseudomonas species, Bacillus Species, Rhizobium Species, Serratia Species and E. coli. These bacterial strains followed the maximum PGPR traits. After proper validation, these bacterial strains could be used for the bio-fertilizer formation for crop production enhancement by improving soil fertility.
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来源期刊
Research Journal of Biotechnology
Research Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
0.60
自引率
0.00%
发文量
192
期刊介绍: We invite you to contribute Research Papers / Short Communications / Review Papers: -In any field of Biotechnology, Biochemistry, Microbiology and Industrial Microbiology, Soil Technology, Agriculture Biotechnology. -in any field related to Food Biotechnology, Nutrition Biotechnology, Genetic Engineering and Commercial Biotechnology. -in any field of Biotechnology related to Drugs and Pharmaceutical products for human beings, animals and plants. -in any field related to Environmental Biotechnolgy, Waste Treatment of Liquids, Soilds and Gases; Sustainability. -in inter-realted field of Chemical Sciences, Biological Sciences, Environmental Sciences and Life Sciences. -in any field related to Biotechnological Engineering, Industrial Biotechnology and Instrumentation. -in any field related to Nano-technology. -in any field related to Plant Biotechnology.
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