人体乳腺癌正位和异位体内模型的移植和生长动态评估

I. Lyashenko, M. Romanova, A. S. Goncharova, D. Khodakova, A. V. Galina, S. Gurova, S. Filippova, Y.S. Shatova
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摘要

研究目的这项研究旨在利用各种生物材料评估乳腺癌异种移植物在正位注射和皮下注射过程中的移植和生长动态,并为进一步研究建立适当的乳腺癌模型。我们使用了从患者身上获取的肿瘤碎块、经认证的乳腺癌细胞系 VT20 - 人类乳腺癌;原发性人类乳腺癌细胞系。我们使用了 36 只 Balb/c Nude 系雌性免疫缺陷小鼠作为受体动物。本项目开发了乳腺癌皮下模型和正位模型。从注射开始观察肿瘤生长 28 天,每周测量肿瘤结节 2 次,直至实验结束。实验结果采用中位数和百分位数进行评估。非参数曼-惠特尼检验用于评估差异的显著性。在这项工作的过程中,确定了肿瘤细胞注入不同部位时的生长动态。皮下注射最成功的是注射经认证的 VT20 株系肿瘤细胞。实验结束时,该组肿瘤结节的中位数为 100.32 立方毫米。分析显示了肿瘤材料正位注射的肿瘤动态,使用护照培养细胞 VT20 和原代培养细胞组的肿瘤结节体积中位数达到了相同的值--149.22 和 148.25 mm³。研究发现,细胞系和细胞悬液注入肿瘤结节后,肿瘤结节的体积明显增大。我们利用各种材料植入方法获得了乳腺癌肿瘤模型,并有可能进一步用于测试新的药理物质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of engraftment and growth dynamics of orthotopic and heterotopic in vivo models of human breast cancer
Purpose of the study. This work was to assess the engraftment and growth dynamics of breast cancer xenografts during orthotopic and subcutaneous injection using various types of biological material, as well as to develop an adequate model of breast cancer for further research.Materials and methods. We used a disaggregated fragment of a tumor obtained from the patient, a certified breast cancer cell line VT20 – human breast carcinoma; a primary human breast carcinoma cell line. Female immunodeficient mice of the Balb/c Nude line in the amount of 36 animals were used as recipient animals. The subcutaneous and orthotopic models of breast cancer were developed in this project. Tumor growth was observed for 28 days from the moment of injection and tumor nodes were measured 2 times a week until the end of the experiment. Results were assessed using medians and percentiles. The nonparametric Mann-Whitney test was used to assess the significance of differences.Results. The dynamics of the growth of tumor cells when injected into various sites was determined in the process of this work. The most successful in terms of a subcutaneous injection was the injection of tumor cells of the certified VT20 line. By the end of the experiment, the median tumor node of this group was 100.32 mm³. The analysis revealed tumor dynamics with orthotopic injection of tumor material, and the median volume of the tumor node in the group with the passport culture cell VT20 and the primary culture cell reached the same value – 149.22 and 148.25. mm³. It was found that both the cell line and the cell suspension were injected into tumor nodes that reached a significantly larger volume when injected orthotopically.Conclusion. We have obtained a tumor model of breast cancer using various methods of material implantation and with the possibility of further use in testing new pharmacological substances.
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