S. Męczyńska-Wielgosz, T. Bartłomiejczyk, I. Grądzka, Sylwester Sommer, Aneta Węgierek-Ciuk, A. Lankoff, Katarzyna Sikorska, M. Wojewódzka, Małgorzata M Dobrzyńska, Marcin Kruszewski
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引用次数: 0
摘要
我们研究了直径为 21 纳米的 Aeroxide P25 二氧化钛纳米粒子(TiO2 NPs)对诱导 DNA 损伤和三种人类细胞系(肝细胞肝癌 HepG2、结直肠腺癌 HT29 和肺癌 A549)长期存活的影响。研究的终点包括:用彗星试验估算的 DNA 断裂情况、用 FPG+ 彗星试验估算的甲酰胺嘧啶糖基化酶(FPG)识别的氧化碱基损伤情况、组蛋白 γH2AX 病灶和微核的频率、细胞凋亡情况、线粒体活性(MTT)试验测定的细胞代谢活性以及集落形成能力测定的长期存活率。每种细胞系的 DNA 断裂和碱基损伤模式都与纳米粒子(NP)浓度和处理时间有关。与未处理的细胞相比,组蛋白 γH2AX 病灶和微核的频率没有增加。与这些结果相同的是,在所有测试的细胞系中都没有发现诱导细胞凋亡的现象。尽管在最初的 72 小时内线粒体活性和细胞存活率略有下降,但报告的实验没有提供 Aeroxide P25 TiO2 NPs 长期体外毒性的证据。
No evidence of the long-term in vitro toxicity of Aeroxide P25 TiO2 nanoparticles in three mammalian cell lines despite the initial reduction of cellular mitochondrial activity
We studied the effects of Aeroxide P25 titanium dioxide nanoparticles (TiO2 NPs) with a diameter of 21 nm on induction of DNA damage and long-term survival of three human cell lines: hepatocellular liver carcinoma HepG2, colorectal adenocarcinoma HT29 and lung carcinoma A549. The endpoints examined were DNA breakage estimated by the comet assay and oxidative base damage recognized by formamide-pyrimidine glycosylase (FPG) estimated with the FPG+ comet assay, frequencies of histone γH2AX foci and micronuclei, apoptosis, cell metabolic activity measured by mitochondrial activity (MTT) assay and long-term survival measured by colony-forming ability. Each cell line had a different pattern of DNA breakage and base damage vs. nanoparticle (NP) concentration and treatment time. There was no increase in the frequencies of histone γH2AX foci and micronuclei as compared to those in the untreated cells. In parallel with these results, no induction of apoptosis has been found in none of the cell lines tested. The reported experiments provided no evidence of the long-term in vitro toxicity of Aeroxide P25 TiO2 NPs, despite a slight decrease in mitochondrial activity and cell survival during the first 72 h.
期刊介绍:
"Nukleonika" is an international peer-reviewed, scientific journal publishing original top quality papers on fundamental, experimental, applied and theoretical aspects of nuclear sciences.
The fields of research include:
radiochemistry, radiation measurements, application of radionuclides in various branches of science and technology, chemistry of f-block elements, radiation chemistry, radiation physics, activation analysis, nuclear medicine, radiobiology, radiation safety, nuclear industrial electronics, environmental protection, radioactive wastes, nuclear technologies in material and process engineering, radioisotope diagnostic methods of engineering objects, nuclear physics, nuclear reactors and nuclear power, reactor physics, nuclear safety, fuel cycle, reactor calculations, nuclear chemical engineering, nuclear fusion, plasma physics etc.