在耶纳戈阿市出售的 Rhynchophorus phoenicis(非洲棕榈象鼻虫幼虫)中进行细菌计数和检测

C. Opara, D. V. Zige
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引用次数: 0

摘要

对来自街头小贩的Rhynchophorus phoenicis(非洲棕榈象鼻虫幼虫)的微生物污染进行了研究。从尼日利亚巴耶尔萨州耶纳戈阿市(Yenagoa Metropolis)的 Akenfa、Ekeki、Kpansia、Opolo、Swali 和 Etegwe 等地的六个摊贩处购买了在街头销售的 R. phoenicis 幼虫。倒平板法用于评估总存活计数(TVC)、总菌落计数(TCC)和总链球菌计数(TSC)。在采用平板计数法之前,先将样品匀浆,浓度从 0.1 毫升到 10-7 毫升不等,但 10-7 毫升的浓度用于活菌落计数,并无菌接种到麦康凯琼脂、营养琼脂和血琼脂上,以鉴定潜在的病原体。结果显示,TVC 从 48.5±5.4a 到 117.5±8.5b,TCC 从 44.0±5.1ac 到 70.0±8.6ab,TSC 从 44.8±4.8a 到 60.0±6。7a 同样,根据形态和生化特征,在所有样品中鉴定出的细菌有金黄色葡萄球菌、胆色葡萄球菌、链球菌、大肠杆菌、沙门氏菌、肠杆菌属、假单胞菌属、变形杆菌属和克雷伯氏菌属。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bacterial Enumeration and Detection in Rhynchophorus phoenicis (African Palm Weevil Larva) Sold within Yenagoa Metropolis
Microbial contamination of Rhynchophorus phoenicis (larva of the African palm weevil) was examined from street vendors. R. phoenicis larvae sold on the street were purchased from six vendors in the following location including Akenfa, Ekeki, Kpansia, Opolo, Swali and Etegwe in Yenagoa Metropolis, Bayelsa State, Nigeria. The pour plate method was used to assess the total viable count (TVC), Total colony count (TCC) and Total streptococcal count (TSC). Samples were homogenized before the plate counting method was applied at respective concentration ranging from 0.1ml-10-7ml, however 10-7ml concentration was used for viable colony counts and aseptically inoculated onto MacConkey agar, Nutrient agar and blood agar for identification of potential pathogens. Results revealed TVC ranging from 48.5±5.4a to 117.5±8.5b, TCC ranging from 44.0±5.1ac to 70.0±8.6ab and TSC ranging from 44.8±4.8a to 60.0±6.7a Similarly bacteria identified in all samples based on morphological and biochemical characteristics were Staphylococcus aureus, Staphylococcus cholermidis, Streptococcus sp, Escherichia coli, Salmonella sp, Enterobacter spp, Pseudomonas spp, Proteus spp and Klebsiellae spp. Street-sold R. phoenicis, can be a source of food poisoning if not handled properly during, handling, processing and hawking.
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