Muharrem Yılmaz, Ahsen IŞIK ÖZGÜVEN, Yıldız Aka Kaçar
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引用次数: 0
摘要
本研究确定了 16 个榛子品种和 64 个基因型的遗传特征。分子特征研究中使用了 SSR 方法。使用了 18 种 SSR 引物。在 SSR 方法中,共获得 118 个条带,其中 115 个具有多态性。在 SSR 法中,总体基因型的相似系数介于 0.12 和 0.98 之间,所使用引物对的多态性率为 98.0%。CAC- C 028位点的多态性信息含量值最低(0.542),CAC- B 113位点的多态性信息含量值最高(0.987)。平均多态性信息含量值为 0.786。SSR位点的总鉴别力值为17.98;CAC- B 020位点的鉴别力值最低(0.748),CAC- A 024 b位点的鉴别力值最高(1.404)。基因型在树枝图上的聚类位置与其地理起源基本相关。这一结果表明,遗传起源对榛子基因型的分类起着重要作用。
Molecular characterization of Turkish hazelnut cultivars and genotypes using SSR markers
This study defines the genetic characterization of 16 hazelnut varieties and 64 genotypes. SSR method was used in molecular characterization studies. 18 SSR primers were used. In the SSR method, 118 bands were obtained, and 115 were polymorphic. The similarity coefficient in overall genotypes were between 0.12 and 0.98 in SSR andthe polymorphism rate of the primer pairs used was calculated as 98.0%. The lowest polymorphism information content value (0.542) was obtained from the CAC- C 028 locus, and the highest polymorphism information content value (0.987) was obtained from the CAC- B 113 locus. The average polymorphism information content value was 0.786. The total discrimination power values of SSR loci were determined as 17.98; the lowest (0.748) discrimination power value was obtained from the CAC- B 020 locus, while the highest (1.404) discrimination power value was obtained from the CAC- A 024 b locus. The genotypes’clustering positions across the dendograms were essentially correlated with their geographic origins. This result shows that genetic origin plays an important role in terms of classification of genotypes in hazelnut.