外周血在 100-500 mGy 剂量范围内的体外辐照下的化学发光特性

A. Fedotova, G. Makarskaya
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摘要

摘要如今,许多研究都致力于确定低剂量电离辐射的放射生物学效应。众所周知,生物细胞中的电离辐射会增加活性氧(ROS)的生成。这项工作的目的是确定在 100-500 mGy 剂量的体外辐射下外周血中 ROS 化学发光动力学的基本参数。这项研究将扩展低剂量电离辐射对农场动物机体组织影响程度的知识。科学新颖性在于,研究成果将有助于预测低剂量辐射对多细胞生物体的辐射生物学效应。该研究属于放射生物学的实用和基础方向。研究方法。对血液样本进行 100-500 mGy 剂量范围内的体外辐射。在 36 通道化学照度计 3604-PC 上记录 ROS 的生成动力学。结果显示在 100-300 mGy 剂量范围内,自由基自发和活化生成的第一个峰值的形成时间加快;在 500 mGy 剂量下,自发和活化生成的初级 ROS 的第一个峰值的形成时间减慢。在低吸收剂量的影响下,初级和次级自由基生成的最大强度下降。在 100-300 mGy 剂量下,原生自由基自发生成受到抑制;在 400 mGy 剂量下,原生 ROS 的抗原激活生成受到抑制。在 100 mGy 剂量下,自发产生的二次 ROS 量减少。在 100-300 mGy 的剂量下,抗原激活的二级自由基产生量减少。500 mGy剂量抑制了血细胞产生原生自由基的反应能力。100-500 mGy 的体外伽马辐射对外周血细胞有负面影响,表现为化学发光反应加快,自发和抗原激活的原生和次生自由基产生的第一个峰值的最大强度降低。因此,原生自由基和次生自由基的产生量也有所减少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemiluminescent characteristics of peripheral blood under in vitro irradiation in the dose range of 100–500 mGy
Abstract. Nowadays a lot of examinations are dedicated to determination of radiobiological effects under low doses of ionising radiation. It is known that ionising radiation in the cells of an organism increases generation of reactive oxygen species (ROS). The purpose of the work is to determine basic parameters of chemiluminescent kinetics of ROS in peripheral blood under in vitro radiation in the range of 100–500 mGy doses. The research will extend the knowledge of the impact degree of ionising radiation in low doses on organism tissue of farm animals. Scientific novelty is that the results of the research will allow to predict radiobiological effects of low doses in a multicellular organism. The research is included in practical and fundamental directions of radiobiology. Research methods. In vitro radiation of blood samples in the range of 100–500 mGy doses. The kinetics of generation of ROS is registered on 36-channel chemiluminometer 3604-PC. Results. The doses in the range of 100–300 mGy accelerated the time of the first peak of spontaneous and activated generation of radicals, under 500 mGy dose the formation of the first peak of spontaneous and activated generation of primary ROS decelerated. Under the impact of low absorbed doses maximum intensity of primary and secondary radicals generation decreased. The inhibition of primary radicals spontaneous production occurred under 100–300 mGy doses, the inhibition of antigen activated generation of primary ROS was under 400 mGy. The reduction of the amount of spontaneous secondary ROS was registered under 100 mGy dose. The decrease of antigen activated secondary radicals production occurred under 100–300 mGy doses. 500 mGy dose inhibited a reaction ability of blood cells to generate primary radicals. The external in vitro gamma radiation in the range of 100–500 mGy had a negative impact on peripheral blood cells showing an acceleration of chemiluminescent reaction as well as decrease of maximum intensity of the first peak of spontaneous and antigen activated primary and secondary radicals production. As a consequence, a decrease of the amount of primary and secondary radicals production was revealed.
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