共同培养有益于可持续农业的多菌种革兰氏阴性接种剂

Beatrice Farda, Fabrizia Pasquarelli, R. Djebaili, D. Spera, M. del Gallo, M. Pellegrini
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引用次数: 0

摘要

农用微生物接种剂由不同的菌株组成。联合体的生产过程包括分别培养纯培养物,然后按适当比例将其混合。然而,在配方中加入多种菌株会增加生产成本。通过开发共培养发酵法,有可能制造出具有正确细菌含量的联合菌株。本研究旨在开发一种具有成本效益的共培养方法,以生产包含四种革兰氏阴性菌(即巴西绿氮青霉、埋伏伯克霍尔德氏菌、重氮葡萄糖醛酸杆菌和血清草霉)适当比例的接种物。研究人员使用 T4 培养基对菌株的特定生长率进行了研究。在 500 mL 烧瓶中对联合发酵过程进行了优化,以达到 9.7-10 Log CFU mL-1 的当量密度。然后,成功地将其扩大到 5 升生物反应器,获得了 9.7-9.9 Log CFU mL-1 的等效密度。这是由革兰氏阴性植物生长促进菌组成的四个多菌株联合体的首次共同配方,为今后评估对可持续农业有用的其他产品铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Co-culturing a multistrain Gram-negative inoculant useful in sustainable agriculture
Microbial-based inoculants for agricultural use consist of different strains. The consortia production process involves growing pure cultures separately and combining them in the appropriate ratio. However, the inclusion of multiple strains in the formulation increases production costs. By developing co-culture fermentations, it is possible to create consortia with the correct bacterial charge. This study aims to develop a cost-effective co-culture approach for producing an inoculum that includes the appropriate ratio of four Gram-negative bacteria, i.e., Azospirillum brasilense, Burkholderia ambifaria, Gluconacetobacter diazotrophicus, and Herbaspirillum seropedicae. The specific growth rates of strains were studied using the T4 medium, previously optimized for their culture. The co-fermentation process was optimized in 500 mL flasks to attain an equivalent density of 9.7-10 Log CFU mL-1. Then, it was successfully scaled up to a 5 L bioreactor, obtaining an equivalent density of 9.7-9.9 CFU mL-1. This first co-formulation of a four multistrain consortium formed by Gram-negative plant growth-promoting bacteria pave the road for future evaluations of other products useful for sustainable agriculture.
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