基质金属蛋白酶-1 作为早期胃癌检测的潜在生物标记及其对胃癌细胞增殖和迁移的影响

Tumor discovery Pub Date : 2024-03-26 DOI:10.36922/td.1973
Ke Yi, Yan Hu, Xiaoli Zhu, Qing Li
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引用次数: 0

摘要

本研究旨在探讨基质金属蛋白酶-1(MMP-1)与早期胃癌(EGC)之间的关系,同时评估MMP-1对胃癌细胞增殖和迁移的影响。为了评估MMP-1表达与EGC之间的关系,研究人员进行了转录组RNA测序和数据库分析。利用荧光定量聚合酶链反应(PCR)检测了临床 EGC 样本和癌旁组织中 MMP-1 表达的差异。在 N87 胃癌细胞中,测定了增殖和迁移相关指标表达的变化。基因测序显示,MMP-1 在早期和晚期胃癌中的表达存在差异。此外,在早期和晚期胃癌组织中观察到 MMP-1 表达增强,与胃癌细胞系的恶性表型呈正相关。荧光定量 PCR 显示,EGC 组织中的 MMP-1 表达明显高于癌旁组织。CCK8和EdU检测表明,MMP-1上调时,N87细胞的增殖显著增加,而下调时则显著减少。划痕试验结果表明,MMP-1 上调时,N87 细胞的迁移能力相应增强,而下调时则减弱。Western 印迹实验显示,MMP-1 上调后,上皮-间质转化相关蛋白 E-cadherin 的表达量减少,而波形蛋白的表达量则显著增加。相反,下调 MMP-1 则会导致相反的结果。总之,MMP-1是诊断EGC的潜在生物标记物,在N87胃癌细胞增殖和迁移的调控中发挥着关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Matrix metalloproteinase-1 as a potential biomarker for early gastric cancer detection and its effect on gastric cancer cell proliferation and migration
The present study aimed to investigate the association between matrix metalloproteinase-1 (MMP-1) and early gastric cancer (EGC), while also evaluating the effect of MMP-1 on gastric cancer cell proliferation and migration. Transcriptome RNA sequencing and database analysis were conducted to assess the relationship between MMP-1 expression and EGC. Differences in MMP-1 expression between clinical EGC samples and paracancerous tissues were detected using fluorescence quantitative polymerase chain reaction (PCR). In N87 gastric cancer cells, changes in proliferation- and migration-related indicator expression were determined. Gene sequencing revealed differential expression of MMP-1 in early and advanced gastric cancers. Furthermore, enhanced MMP-1 expression was observed in early and advanced gastric cancer tissues, exhibiting a positive correlation with the malignant phenotype in gastric cancer cell lines. Fluorescence quantitative PCR revealed considerably higher MMP-1 expression in EGC tissues than in paracancerous tissues. CCK8 and EdU assays demonstrated a significant increase in N87 cell proliferation on MMP-1 upregulation and a decrease on its downregulation. The scratch assay results demonstrated a corresponding enhancement in N87 cell migratory capacity with MMP-1 upregulation, which was attenuated on its downregulation. Western blot experiments revealed a decrease in the expression of the epithelial-mesenchymal transition-related protein E-cadherin after MMP-1 upregulation, while vimentin expression significantly increased. Conversely, the downregulation of MMP-1 led to opposite outcomes. Overall, MMP-1 emerges as a potential biomarker for EGC diagnosis and plays a crucial role in the regulation of N87 gastric cancer cell proliferation and migration.
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