{"title":"探索粘蛋白对脂质双分子层的影响：洞察相依赖性相互作用","authors":"Jin-WonPark","doi":"10.56557/bn/2024/v44i11983","DOIUrl":null,"url":null,"abstract":"Changes in the behavior of the prepared lipid bilayer according to each phase of the layer, induced by mucin incorporation, were investigated. 8-Aminonaphtalene-1,3,6-trisulfonic acid disodium salt (ANTS) was encapsulated only in the water-soluble interior of the vesicle. p-xylene-bis(N-pyridinium bromide) (DPX) settled only outside of the vesicles to quench the ANTS. The calibration was established for the fluorescence with the encapsulated ANTS separated from the DPX (100%) and the ANTS mixed with DPX (0%). The changes were found due to the layer phase over the ratio of mucin to lipid. The changes are determined by the stability of the vesicles.","PeriodicalId":490896,"journal":{"name":"BIONATURE","volume":"11 8","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring the Influence of Mucin on Lipid Bilayers: Insights into Phase-Dependent Interactions\",\"authors\":\"Jin-WonPark\",\"doi\":\"10.56557/bn/2024/v44i11983\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Changes in the behavior of the prepared lipid bilayer according to each phase of the layer, induced by mucin incorporation, were investigated. 8-Aminonaphtalene-1,3,6-trisulfonic acid disodium salt (ANTS) was encapsulated only in the water-soluble interior of the vesicle. p-xylene-bis(N-pyridinium bromide) (DPX) settled only outside of the vesicles to quench the ANTS. The calibration was established for the fluorescence with the encapsulated ANTS separated from the DPX (100%) and the ANTS mixed with DPX (0%). The changes were found due to the layer phase over the ratio of mucin to lipid. The changes are determined by the stability of the vesicles.\",\"PeriodicalId\":490896,\"journal\":{\"name\":\"BIONATURE\",\"volume\":\"11 8\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BIONATURE\",\"FirstCategoryId\":\"0\",\"ListUrlMain\":\"https://doi.org/10.56557/bn/2024/v44i11983\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BIONATURE","FirstCategoryId":"0","ListUrlMain":"https://doi.org/10.56557/bn/2024/v44i11983","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
研究了在加入粘蛋白的诱导下，所制备的脂质双分子层各阶段的行为变化。8-氨基萘-1,3,6-三磺酸二钠盐（ANTS）只被包裹在囊泡的水溶性内部，对二亚甲苯双（N-吡啶溴化物）（DPX）只沉淀在囊泡外部以淬灭 ANTS。在封装的 ANTS 与 DPX 分离（100%）和 ANTS 与 DPX 混合（0%）的情况下，对荧光进行了校准。发现变化是由于层相随粘蛋白和脂质的比例变化而产生的。这些变化取决于囊泡的稳定性。
Exploring the Influence of Mucin on Lipid Bilayers: Insights into Phase-Dependent Interactions
Changes in the behavior of the prepared lipid bilayer according to each phase of the layer, induced by mucin incorporation, were investigated. 8-Aminonaphtalene-1,3,6-trisulfonic acid disodium salt (ANTS) was encapsulated only in the water-soluble interior of the vesicle. p-xylene-bis(N-pyridinium bromide) (DPX) settled only outside of the vesicles to quench the ANTS. The calibration was established for the fluorescence with the encapsulated ANTS separated from the DPX (100%) and the ANTS mixed with DPX (0%). The changes were found due to the layer phase over the ratio of mucin to lipid. The changes are determined by the stability of the vesicles.
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