利用枯草杆菌 RIK1285 细胞在细胞外表达淀粉样芽孢杆菌 mdc1974 株的α-淀粉酶基因

BULLETIN OF HIGH TECHNOLOGY Pub Date : 2024-03-27 DOI:10.56243/18294898-2024.1-15

Tigran Soghomonyan

{"title":"利用枯草杆菌 RIK1285 细胞在细胞外表达淀粉样芽孢杆菌 mdc1974 株的α-淀粉酶基因","authors":"Tigran Soghomonyan","doi":"10.56243/18294898-2024.1-15","DOIUrl":null,"url":null,"abstract":"The alpha-amylase gene from Bacillus amyloliquefaciens MDC1974 strain was molecularly cloned into the E. coli/B. subtilis pBE-S shuttle vector and subsequently expressed extracellularly by the recipient strain Bacillus subtilis RIK1285, which exhibited low protease activity. As a result of optimizing fermentation conditions, a secretion of alpha-amylase with an activity of 1400 units per mL was achieved.","PeriodicalId":472444,"journal":{"name":"BULLETIN OF HIGH TECHNOLOGY","volume":"72 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"EXTRACELLULAR EXPRESSION OF THE ALPHA-AMYLASE GENE FROM BACILLUS AMYLOLIQUEFACIENS MDC1974 STRAIN USING BACILLUS SUBTILIS RIK1285 CELLS\",\"authors\":\"Tigran Soghomonyan\",\"doi\":\"10.56243/18294898-2024.1-15\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The alpha-amylase gene from Bacillus amyloliquefaciens MDC1974 strain was molecularly cloned into the E. coli/B. subtilis pBE-S shuttle vector and subsequently expressed extracellularly by the recipient strain Bacillus subtilis RIK1285, which exhibited low protease activity. As a result of optimizing fermentation conditions, a secretion of alpha-amylase with an activity of 1400 units per mL was achieved.\",\"PeriodicalId\":472444,\"journal\":{\"name\":\"BULLETIN OF HIGH TECHNOLOGY\",\"volume\":\"72 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BULLETIN OF HIGH TECHNOLOGY\",\"FirstCategoryId\":\"0\",\"ListUrlMain\":\"https://doi.org/10.56243/18294898-2024.1-15\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BULLETIN OF HIGH TECHNOLOGY","FirstCategoryId":"0","ListUrlMain":"https://doi.org/10.56243/18294898-2024.1-15","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

将淀粉芽孢杆菌 MDC1974 菌株的α-淀粉酶基因分子克隆到大肠杆菌/枯草杆菌 pBE-S 穿梭载体中，然后用具有低蛋白酶活性的枯草杆菌 RIK1285 菌株进行胞外表达。通过优化发酵条件，α-淀粉酶的分泌活性达到了每毫升 1400 个单位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

EXTRACELLULAR EXPRESSION OF THE ALPHA-AMYLASE GENE FROM BACILLUS AMYLOLIQUEFACIENS MDC1974 STRAIN USING BACILLUS SUBTILIS RIK1285 CELLS

The alpha-amylase gene from Bacillus amyloliquefaciens MDC1974 strain was molecularly cloned into the E. coli/B. subtilis pBE-S shuttle vector and subsequently expressed extracellularly by the recipient strain Bacillus subtilis RIK1285, which exhibited low protease activity. As a result of optimizing fermentation conditions, a secretion of alpha-amylase with an activity of 1400 units per mL was achieved.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

BULLETIN OF HIGH TECHNOLOGY

自引率

0.00%

发文量