{"title":"促炎细胞因子 TNF-α (rs1800629) 和 IL-6 (rs1800795) 在北印度女性多囊卵巢综合征发病机制中的作用","authors":"Parneet Kaur, Khushpreet Kaur, Rajinder Kaur","doi":"10.18203/2320-1770.ijrcog20240796","DOIUrl":null,"url":null,"abstract":"Background: This study aimed to determine whether TNF-α and IL-6 gene polymorphism, their epistatic effects and haplotypes confer pathogenesis of PCOS in north Indian females.\nMethods: A case-control study comprising DNA samples of 401 females (200 PCOS cases and 201 controls) of reproductive age. All the subjects were genotyped for TNF-α (-308 G/A) and IL-6 (-174 G/C) genes by tetra-primer ARMS PCR.\nResults: There were 41.5% PCOS females revealing hirsutism, 45.5% acne and 36% alopecia. High BMI (p=0.008) and W/H ratio (p=0.0001) was observed among PCOS cases. Frequency of minor allele A for -308 G/A TNF-α was significantly higher in PCOS cases than controls indicating 1.4 fold increased risk for PCOS (p=0.05, OR=1.41, 95% CI=1.00-1.99). -174 G/C IL-6 gene was in association with the decreased risk of PCOS. The epistatic effects of all the possible combinations of both the SNPs shows statistically significant differences (Interaction p value=0.014) indicating modulating effects of TNF-α and IL-6 polymorphism in response to PCOS. Haplotype HT3 AG (p=0.003, OR=2.22, 95% CI=1.31-3.78) was associated with increased risk of PCOS.\nConclusions: The present findings suggest that TNF-α and IL-6 might contribute to pathogenesis of PCOS in north Indian females irrespective of a polymorphism of TNF-α (-308 G/A) and IL-6 (-174 G/C) genes.","PeriodicalId":14225,"journal":{"name":"International journal of reproduction, contraception, obstetrics and gynecology","volume":"52 10","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Role of proinflammatory cytokines TNF-α (rs1800629) and IL-6 (rs1800795) in the pathogenesis of polycystic ovarian syndrome in North Indian females\",\"authors\":\"Parneet Kaur, Khushpreet Kaur, Rajinder Kaur\",\"doi\":\"10.18203/2320-1770.ijrcog20240796\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: This study aimed to determine whether TNF-α and IL-6 gene polymorphism, their epistatic effects and haplotypes confer pathogenesis of PCOS in north Indian females.\\nMethods: A case-control study comprising DNA samples of 401 females (200 PCOS cases and 201 controls) of reproductive age. All the subjects were genotyped for TNF-α (-308 G/A) and IL-6 (-174 G/C) genes by tetra-primer ARMS PCR.\\nResults: There were 41.5% PCOS females revealing hirsutism, 45.5% acne and 36% alopecia. High BMI (p=0.008) and W/H ratio (p=0.0001) was observed among PCOS cases. Frequency of minor allele A for -308 G/A TNF-α was significantly higher in PCOS cases than controls indicating 1.4 fold increased risk for PCOS (p=0.05, OR=1.41, 95% CI=1.00-1.99). -174 G/C IL-6 gene was in association with the decreased risk of PCOS. The epistatic effects of all the possible combinations of both the SNPs shows statistically significant differences (Interaction p value=0.014) indicating modulating effects of TNF-α and IL-6 polymorphism in response to PCOS. Haplotype HT3 AG (p=0.003, OR=2.22, 95% CI=1.31-3.78) was associated with increased risk of PCOS.\\nConclusions: The present findings suggest that TNF-α and IL-6 might contribute to pathogenesis of PCOS in north Indian females irrespective of a polymorphism of TNF-α (-308 G/A) and IL-6 (-174 G/C) genes.\",\"PeriodicalId\":14225,\"journal\":{\"name\":\"International journal of reproduction, contraception, obstetrics and gynecology\",\"volume\":\"52 10\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of reproduction, contraception, obstetrics and gynecology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18203/2320-1770.ijrcog20240796\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of reproduction, contraception, obstetrics and gynecology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18203/2320-1770.ijrcog20240796","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Role of proinflammatory cytokines TNF-α (rs1800629) and IL-6 (rs1800795) in the pathogenesis of polycystic ovarian syndrome in North Indian females
Background: This study aimed to determine whether TNF-α and IL-6 gene polymorphism, their epistatic effects and haplotypes confer pathogenesis of PCOS in north Indian females.
Methods: A case-control study comprising DNA samples of 401 females (200 PCOS cases and 201 controls) of reproductive age. All the subjects were genotyped for TNF-α (-308 G/A) and IL-6 (-174 G/C) genes by tetra-primer ARMS PCR.
Results: There were 41.5% PCOS females revealing hirsutism, 45.5% acne and 36% alopecia. High BMI (p=0.008) and W/H ratio (p=0.0001) was observed among PCOS cases. Frequency of minor allele A for -308 G/A TNF-α was significantly higher in PCOS cases than controls indicating 1.4 fold increased risk for PCOS (p=0.05, OR=1.41, 95% CI=1.00-1.99). -174 G/C IL-6 gene was in association with the decreased risk of PCOS. The epistatic effects of all the possible combinations of both the SNPs shows statistically significant differences (Interaction p value=0.014) indicating modulating effects of TNF-α and IL-6 polymorphism in response to PCOS. Haplotype HT3 AG (p=0.003, OR=2.22, 95% CI=1.31-3.78) was associated with increased risk of PCOS.
Conclusions: The present findings suggest that TNF-α and IL-6 might contribute to pathogenesis of PCOS in north Indian females irrespective of a polymorphism of TNF-α (-308 G/A) and IL-6 (-174 G/C) genes.