评估姜黄(姜科植物)对实验室小鼠中伯格氏疟原虫的抗疟活性。

Q4 Immunology and Microbiology
A. Dawet, C. Golnaan, K. Yusuf, E.T. Lengnen, N. B. Kamji, D. Yakubu
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引用次数: 0

摘要

疟疾是全球发病率和死亡率的主要原因,尤其是在发展中国家和撒哈拉以南非洲地区,那里是疟疾病例和死亡人数最多的地方。疟原虫对大多数抗疟疾药物具有抗药性,加上一些药物的高成本和毒性作用,为寻找新的低成本有效抗疟疾化合物带来了挑战。本研究旨在确定莪术对啮齿类动物中的伯格氏疟原虫的抗疟活性。研究共使用了 110 只体重为 18-30 克的瑞士白化小鼠:其中 35 只用于毒性试验,75 只用于抗疟研究。每次试验给 25 只小鼠接种对药物敏感的 Nk65 伯格氏疟原虫,将其分成五组,每组五只,每组施用以下药物中的一种:120 毫克/千克乙醇提取物、120 毫克/千克水提取物、120 毫克/千克正己烷提取物、1.2 毫克/千克乙胺嘧啶或 5 毫克/千克氯喹(阳性对照)和 0.2 毫升生理盐水(阴性对照)。致死剂量浓度高于 1500 毫克/千克,提取物显示出显著的抗疟活性(P<0.05),在治疗试验中,乙醇处理组的抑制百分比最高(67.49%),其次是在抑制试验中给水处理组,抑制百分比为 65.03%,在预防试验中给正己烷处理组,抑制百分比为 64.98%。与标准药物(氯喹 5 毫克/公斤或嘧啶 1.2 毫克/公斤/天)相比,不同溶剂萃取物的抗疟活性略有不同,但没有完全清除寄生虫的记录。龙牙草具有相当强的抗疟活性,可用于疟疾治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the antiplasmodial activity of Curcuma longa (turmeric - Zingiberaceae) on Plasmodium berghei in laboratory mice.
Malaria is a leading cause of morbidity and mortality worldwide, especially in developing countries and in sub-Saharan Africa where most malaria cases and deaths occur. The resistance of malaria parasites to most anti-malaria drugs, coupled with the high cost and the toxic effects of some drugs has posed a challenge for the search of new effective anti-malarial compounds of low cost. The study aims to determine the antiplasmodial activity of Curcuma longa against Plasmodium berghei in rodents. A total of 110 Swiss albino mice weighing 18-30 grams were used for the study: 35 for the toxicity test and 75 for the antimalarial study. For each test, 25 mice were inoculated with drug-sensitive Nk65 Plasmodium berghei and divided into five groups of five animals each and each group was administered one of the following: 120mg/kg of ethanol extract, 120mg/kg water extract, 120mg/kg nHexane extracts of C. longa, 1.2mg/kg of pyrimethamine or 5mg/kg of Chloroquine (positive control) and 0.2mls of normal saline (negative control). The lethal dose concentration was above 1500mg/kg and the extracts showed significant (P<0.05) antimalarial activity with the highest percentage inhibition (67.49%) recorded in the group treated with ethanol in the curative test, followed by the group given water in the suppressive test with (65.03%) and nHexane in the prophylactic test with percentage inhibition of 64.98%. There was a slight difference in the antimalarial activities of the extracts of different solvents which all had lower activities compared with the standard drugs (Chloroquine administered at 5mg/kg or pyrimethamine, 1.2mg/kg/day) but no total clearance of the parasite was recorded. C. longa possesses considerable antiplasmodial activity, which can be exploited in malaria therapy.
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来源期刊
Nigerian Journal of Parasitology
Nigerian Journal of Parasitology Medicine-Infectious Diseases
CiteScore
0.20
自引率
0.00%
发文量
43
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