钙凝蛋白在盲肠肌内的表达:观察及与阑尾炎的假定相关性。

IF 1.3 4区 医学 Q3 PATHOLOGY
Pediatric and Developmental Pathology Pub Date : 2024-05-01 Epub Date: 2024-03-28 DOI:10.1177/10935266241235507
Raj P Kapur
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引用次数: 0

摘要

背景:在盲肠固有肌平滑肌细胞中意外观察到钙网蛋白免疫反应,这促使我们对钙网蛋白的表达及其与蚓部盲肠周围推进收缩活动的可能关系进行更详细的研究:方法:采用免疫组织化学和 RNA 原位杂交技术分析了 33 名患者(年龄从妊娠中期的胎儿到成年人不等)的肠道样本以及一些可能相关的动物模型中的钙网蛋白表达情况。为了比较钙网蛋白与平滑肌和 Cajal 间质细胞标记物的定位,还进行了双重免疫标记:结果:在人类盲肠、阑尾底部和升结肠近端最内层的肌间平滑肌层中持续观察到钙黄绿素的表达,但在肠道的其他部位没有观察到钙黄绿素的表达。钙网蛋白阳性的平滑肌细胞并不同时表达位于邻近卡贾尔间质细胞的标记物。在没有盲肠的小鼠和猕猴的盲肠中,以及在兔子的盲肠和盲肠中都没有检测到钙网蛋白的免疫反应:结论:在盲肠平滑肌细胞中局部表达钙调蛋白可能会降低钙介导的近端结肠逆行推进收缩的可能性,并抑制盲肠中促炎性粪便淤积。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression of Calretinin in the Cecal Muscularis Interna: Observation and Hypothetical Relevance to Appendicitis.

Background: The unexpected observation of calretinin immunoreactivity in smooth muscle cells in the muscularis propria of the cecum led to a more detailed examination of calretinin expression and its possible relationship to propulsive contractile activity around the vermiform appendix.

Methods: Immunohistochemistry and RNA in situ hybridization were performed to analyze calretinin expression in intestinal samples from 33 patients at ages ranging from mid-gestation fetuses to adults, as well as in some potentially relevant animal models. Dual immunolabeling was done to compare calretinin localization with markers of smooth muscle and interstitial cells of Cajal.

Results: Calretinin expression was observed consistently in the innermost smooth muscle layers of the muscularis interna in the human cecum, appendiceal base, and proximal ascending colon, but not elsewhere in the intestinal tract. Calretinin-positive smooth muscle cells did not co-express markers located in adjacent interstitial cells of Cajal. Muscular calretinin immunoreactivity was not detected in the ceca of mice or macaques, species which lack appendices, nor in the rabbit cecum or appendix.

Conclusions: Localized expression of calretinin in cecal smooth muscle cells may reduce the likelihood of retrograde, calcium-mediated propulsive contractions from the proximal colon and suppress pro-inflammatory fecal stasis in the appendix.

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来源期刊
CiteScore
3.70
自引率
5.30%
发文量
59
审稿时长
6-12 weeks
期刊介绍: The Journal covers the spectrum of disorders of early development (including embryology, placentology, and teratology), gestational and perinatal diseases, and all diseases of childhood. Studies may be in any field of experimental, anatomic, or clinical pathology, including molecular pathology. Case reports are published only if they provide new insights into disease mechanisms or new information.
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