过氧化物酶的硅学鉴定和表达分析

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ho Am Jang, Sung Min Ku, Jae Hui Kim, Sang Mok Jung, Jongdae Lee, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo
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引用次数: 0

摘要

人类在农业、城市化和工业化方面的进步导致了各种形式的环境污染,包括重金属污染。昆虫作为一种适应性很强的生物,可以在各种环境压力下生存,而环境压力会诱发氧化损伤并损害抗氧化系统。为了研究褐飞虱的过氧化物酶(POX)家族,我们鉴定了两种POX,即TmPOX-iso1和TmPOX-iso2。TmPOX-iso1和TmPOX-iso2的全长cDNA序列分别由一个1815 bp的开放阅读框和一个2229 bp的开放阅读框组成,前者编码605个氨基酸,后者编码743个氨基酸。在五个不同的昆虫目中发现了 TmPOX-iso1 和 TmPOX-iso2 的同源物。在系统发生树分析中,TmPOX-iso1与蓖麻的预测POX蛋白聚类,TmPOX-iso2与蓖麻的POX前体蛋白聚类。在发育过程中,TmPox-iso1的最高表达水平出现在蛹的前期,而TmPox-iso2的表达则出现在蛹的前期和4天的蛹期。TmPox-iso1 主要在幼虫肠道早期和晚期表达,而 TmPox-iso2 mRNA 在脂肪体和 Malpighian 小管中表达较多。氯化镉处理组中,TmPox-iso1的表达量在3小时后增加,然后在24小时前下降,而氯化锌处理组中,TmPox-iso1的表达量在处理后24小时达到峰值。两个处理组的 TmPox-iso2 表达量在处理 24 小时后都有所增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In silico identification and expression analyses of peroxidases in Tenebrio molitor

In silico identification and expression analyses of peroxidases in Tenebrio molitor

Human advancements in agriculture, urbanization, and industrialization have led to various forms of environmental pollution, including heavy metal pollution. Insects, as highly adaptable organisms, can survive under various environmental stresses, which induce oxidative damage and impair antioxidant systems. To investigate the peroxidase (POX) family in Tenebrio molitor, we characterized two POXs, namely TmPOX-iso1 and TmPOX-iso2. The full-length cDNA sequences of TmPox-iso1 and TmPox-iso2 respectively consisted of an open reading frame of 1815 bp encoding 605 amino acids and an open reading frame of 2229 bp encoding 743 amino acids. TmPOX-iso1 and TmPOX-iso2 homologs were found in five distinct insect orders. In the phylogenetic tree analysis, TmPOX-iso1 was clustered with the predicted POX protein of T. castaneum, and TmPOX-iso2 was clustered with the POX precursor protein of T. castaneum. During development, the highest expression level of TmPox-iso1 was observed in the pre-pupal stage, while that of TmPox-iso2 expression were observed in the pre-pupal and 4-day pupal stages. TmPox-iso1 was primarily expressed in the early and late larval gut, while TmPox-iso2 mRNA expression was higher in the fat bodies and Malpighian tubules. In response to cadmium chloride treatment, TmPox-iso1 expression increased at 3 hours and then declined until 24 hours, while in the zinc chloride-treated group, TmPox-iso1 expression peaked 24 hours after the treatment. Both treated groups showed increases in TmPox-iso2 expression 24 hours after the treatments.

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来源期刊
Genes & genomics
Genes & genomics 生物-生化与分子生物学
CiteScore
3.70
自引率
4.80%
发文量
131
审稿时长
6-12 weeks
期刊介绍: Genes & Genomics is an official journal of the Korean Genetics Society (http://kgenetics.or.kr/). Although it is an official publication of the Genetics Society of Korea, membership of the Society is not required for contributors. It is a peer-reviewed international journal publishing print (ISSN 1976-9571) and online version (E-ISSN 2092-9293). It covers all disciplines of genetics and genomics from prokaryotes to eukaryotes from fundamental heredity to molecular aspects. The articles can be reviews, research articles, and short communications.
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