Ho Am Jang, Sung Min Ku, Jae Hui Kim, Sang Mok Jung, Jongdae Lee, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo
{"title":"过氧化物酶的硅学鉴定和表达分析","authors":"Ho Am Jang, Sung Min Ku, Jae Hui Kim, Sang Mok Jung, Jongdae Lee, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo","doi":"10.1007/s13258-024-01498-7","DOIUrl":null,"url":null,"abstract":"<p>Human advancements in agriculture, urbanization, and industrialization have led to various forms of environmental pollution, including heavy metal pollution. Insects, as highly adaptable organisms, can survive under various environmental stresses, which induce oxidative damage and impair antioxidant systems. To investigate the peroxidase (POX) family in <i>Tenebrio molitor</i>, we characterized two POXs, namely <i>Tm</i>POX-iso1 and <i>Tm</i>POX-iso2. The full-length cDNA sequences of <i>TmPox-iso1</i> and <i>TmPox-iso2</i> respectively consisted of an open reading frame of 1815 bp encoding 605 amino acids and an open reading frame of 2229 bp encoding 743 amino acids. <i>Tm</i>POX-iso1 and <i>Tm</i>POX-iso2 homologs were found in five distinct insect orders. In the phylogenetic tree analysis, <i>Tm</i>POX-iso1 was clustered with the predicted POX protein of <i>T. castaneum</i>, and <i>Tm</i>POX-iso2 was clustered with the POX precursor protein of <i>T. castaneum</i>. During development, the highest expression level of <i>TmPox-iso1</i> was observed in the pre-pupal stage, while that of <i>TmPox-iso2</i> expression were observed in the pre-pupal and 4-day pupal stages. <i>TmPox-iso1</i> was primarily expressed in the early and late larval gut, while <i>TmPox-iso2</i> mRNA expression was higher in the fat bodies and Malpighian tubules. In response to cadmium chloride treatment, <i>TmPox-iso1</i> expression increased at 3 hours and then declined until 24 hours, while in the zinc chloride-treated group, <i>TmPox-iso1</i> expression peaked 24 hours after the treatment. Both treated groups showed increases in <i>TmPox-iso2</i> expression 24 hours after the treatments.</p>","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":"178 1","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In silico identification and expression analyses of peroxidases in Tenebrio molitor\",\"authors\":\"Ho Am Jang, Sung Min Ku, Jae Hui Kim, Sang Mok Jung, Jongdae Lee, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo\",\"doi\":\"10.1007/s13258-024-01498-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Human advancements in agriculture, urbanization, and industrialization have led to various forms of environmental pollution, including heavy metal pollution. Insects, as highly adaptable organisms, can survive under various environmental stresses, which induce oxidative damage and impair antioxidant systems. To investigate the peroxidase (POX) family in <i>Tenebrio molitor</i>, we characterized two POXs, namely <i>Tm</i>POX-iso1 and <i>Tm</i>POX-iso2. The full-length cDNA sequences of <i>TmPox-iso1</i> and <i>TmPox-iso2</i> respectively consisted of an open reading frame of 1815 bp encoding 605 amino acids and an open reading frame of 2229 bp encoding 743 amino acids. <i>Tm</i>POX-iso1 and <i>Tm</i>POX-iso2 homologs were found in five distinct insect orders. In the phylogenetic tree analysis, <i>Tm</i>POX-iso1 was clustered with the predicted POX protein of <i>T. castaneum</i>, and <i>Tm</i>POX-iso2 was clustered with the POX precursor protein of <i>T. castaneum</i>. During development, the highest expression level of <i>TmPox-iso1</i> was observed in the pre-pupal stage, while that of <i>TmPox-iso2</i> expression were observed in the pre-pupal and 4-day pupal stages. <i>TmPox-iso1</i> was primarily expressed in the early and late larval gut, while <i>TmPox-iso2</i> mRNA expression was higher in the fat bodies and Malpighian tubules. In response to cadmium chloride treatment, <i>TmPox-iso1</i> expression increased at 3 hours and then declined until 24 hours, while in the zinc chloride-treated group, <i>TmPox-iso1</i> expression peaked 24 hours after the treatment. Both treated groups showed increases in <i>TmPox-iso2</i> expression 24 hours after the treatments.</p>\",\"PeriodicalId\":12675,\"journal\":{\"name\":\"Genes & genomics\",\"volume\":\"178 1\",\"pages\":\"\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-03-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genes & genomics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s13258-024-01498-7\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genes & genomics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s13258-024-01498-7","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
In silico identification and expression analyses of peroxidases in Tenebrio molitor
Human advancements in agriculture, urbanization, and industrialization have led to various forms of environmental pollution, including heavy metal pollution. Insects, as highly adaptable organisms, can survive under various environmental stresses, which induce oxidative damage and impair antioxidant systems. To investigate the peroxidase (POX) family in Tenebrio molitor, we characterized two POXs, namely TmPOX-iso1 and TmPOX-iso2. The full-length cDNA sequences of TmPox-iso1 and TmPox-iso2 respectively consisted of an open reading frame of 1815 bp encoding 605 amino acids and an open reading frame of 2229 bp encoding 743 amino acids. TmPOX-iso1 and TmPOX-iso2 homologs were found in five distinct insect orders. In the phylogenetic tree analysis, TmPOX-iso1 was clustered with the predicted POX protein of T. castaneum, and TmPOX-iso2 was clustered with the POX precursor protein of T. castaneum. During development, the highest expression level of TmPox-iso1 was observed in the pre-pupal stage, while that of TmPox-iso2 expression were observed in the pre-pupal and 4-day pupal stages. TmPox-iso1 was primarily expressed in the early and late larval gut, while TmPox-iso2 mRNA expression was higher in the fat bodies and Malpighian tubules. In response to cadmium chloride treatment, TmPox-iso1 expression increased at 3 hours and then declined until 24 hours, while in the zinc chloride-treated group, TmPox-iso1 expression peaked 24 hours after the treatment. Both treated groups showed increases in TmPox-iso2 expression 24 hours after the treatments.
期刊介绍:
Genes & Genomics is an official journal of the Korean Genetics Society (http://kgenetics.or.kr/). Although it is an official publication of the Genetics Society of Korea, membership of the Society is not required for contributors. It is a peer-reviewed international journal publishing print (ISSN 1976-9571) and online version (E-ISSN 2092-9293). It covers all disciplines of genetics and genomics from prokaryotes to eukaryotes from fundamental heredity to molecular aspects. The articles can be reviews, research articles, and short communications.