Matheus Mendonça de Souza Marques, Isabella de Oliveira Silva, Layara Alexandre Bessa, Luciana Cristina Vitorino
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Thus, we studied the synthesis of photosynthetic pigments, pattern of gas exchange, and photochemistry of cotton plants subjected to inoculation with <i>Diaporthe ueckerae</i> via root and leaf at three different phenological stages (vegetative, reproductive, and maturation). Additionally, we histopathologically analyzed infected leaves using electron microscopy to study the process of leaf colonization by this endophytic fungus. We evidenced that <i>D. ueckerae</i> inoculation negatively affected the synthesis of photosynthetic pigments in plants at vegetative and reproductive stages. Moreover, inoculation also negatively affected the photosynthetic rate and carboxylation efficiency of these plants. We also found that the presence of the endophyte increased transpiration and decreased water use efficiency in the plants. Furthermore, foliar inoculation negatively affected stomatal conductance, whereas inoculation via leaf or root reduced the photochemical performance of cotton. We also observed that <i>D. ueckerae</i> colonizes the leaf tissues of <i>G. hirsutum</i> via glandular trichomes and forces penetration into the epidermis using appressoria, and the plant responds by closing the stomata. The observed physiological alterations are indicative of biotic stress, confirming the hypothesis that <i>D. ueckerae</i> may be an opportunistic phytopathogen for cotton plants.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"26 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Opportunistic pathogenicity observed for the endophytic fungus Diaporthe ueckerae on Gossypium hirsutum plants\",\"authors\":\"Matheus Mendonça de Souza Marques, Isabella de Oliveira Silva, Layara Alexandre Bessa, Luciana Cristina Vitorino\",\"doi\":\"10.1007/s42161-024-01637-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Cotton crops are routinely threatened by emerging fungal diseases. Fungal endophytes also can be considered latent phytopathogens. 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引用次数: 0
摘要
棉花作物经常受到新出现的真菌病害的威胁。真菌内生菌也可被视为潜在的植物病原体。在这项研究中,我们测试了一种假设,即从棉花(Gossypium hirsutum)枯萎叶片中分离出的一种内生菌株 Diaporthe 可引发这种油料植物的生物胁迫生理效应。我们还评估了内生菌与棉花叶片正面菌丝相互作用的组织病理学方面。因此,我们研究了棉花植株在三个不同物候期(营养期、生殖期和成熟期)通过根部和叶片接种 Diaporthe ueckerae 后光合色素的合成、气体交换模式和光化学。此外,我们还利用电子显微镜对受感染的叶片进行了组织病理学分析,以研究这种内生真菌在叶片上的定殖过程。我们发现,接种 D. ueckerae 会对植物营养期和生殖期光合色素的合成产生负面影响。此外,接种还对这些植物的光合速率和羧化效率产生了负面影响。我们还发现,内生菌的存在增加了植物的蒸腾作用,降低了水分利用效率。此外,叶面接种会对气孔导度产生负面影响,而通过叶片或根部接种则会降低棉花的光化学性能。我们还观察到,D. ueckerae 通过腺毛在 G. hirsutum 的叶片组织中定植,并利用附着体侵入表皮,植物则通过关闭气孔做出反应。观察到的生理变化表明存在生物胁迫,证实了 D. ueckerae 可能是棉花植物的一种机会性植物病原菌的假设。
Opportunistic pathogenicity observed for the endophytic fungus Diaporthe ueckerae on Gossypium hirsutum plants
Cotton crops are routinely threatened by emerging fungal diseases. Fungal endophytes also can be considered latent phytopathogens. In this study we tested the hypothesis that an endophytic strain of Diaporthe, isolated from chlorotic leaves of cotton (Gossypium hirsutum), could trigger physiological effects of biotic stress in this oilseed plant. We also assessed the histopathological aspects of the mycelial interaction of the endophyte with the adaxial surface of G. hirsutum leaves. Thus, we studied the synthesis of photosynthetic pigments, pattern of gas exchange, and photochemistry of cotton plants subjected to inoculation with Diaporthe ueckerae via root and leaf at three different phenological stages (vegetative, reproductive, and maturation). Additionally, we histopathologically analyzed infected leaves using electron microscopy to study the process of leaf colonization by this endophytic fungus. We evidenced that D. ueckerae inoculation negatively affected the synthesis of photosynthetic pigments in plants at vegetative and reproductive stages. Moreover, inoculation also negatively affected the photosynthetic rate and carboxylation efficiency of these plants. We also found that the presence of the endophyte increased transpiration and decreased water use efficiency in the plants. Furthermore, foliar inoculation negatively affected stomatal conductance, whereas inoculation via leaf or root reduced the photochemical performance of cotton. We also observed that D. ueckerae colonizes the leaf tissues of G. hirsutum via glandular trichomes and forces penetration into the epidermis using appressoria, and the plant responds by closing the stomata. The observed physiological alterations are indicative of biotic stress, confirming the hypothesis that D. ueckerae may be an opportunistic phytopathogen for cotton plants.
期刊介绍:
The Journal of Plant Pathology (JPP or JPPY) is the main publication of the Italian Society of Plant Pathology (SiPAV), and publishes original contributions in the form of full-length papers, short communications, disease notes, and review articles on mycology, bacteriology, virology, phytoplasmatology, physiological plant pathology, plant-pathogeninteractions, post-harvest diseases, non-infectious diseases, and plant protection. In vivo results are required for plant protection submissions. Varietal trials for disease resistance and gene mapping are not published in the journal unless such findings are already employed in the context of strategic approaches for disease management. However, studies identifying actual genes involved in virulence are pertinent to thescope of the Journal and may be submitted. The journal highlights particularly timely or novel contributions in its Editors’ choice section, to appear at the beginning of each volume. Surveys for diseases or pathogens should be submitted as "Short communications".