{"title":"甲基组和转录组的整合分析揭示了核酸分枝杆菌在喉癌中的表观遗传调控。","authors":"Xiaohui Yuan, Hui-Ching Lau, Huiying Huang, Chi-Yao Hsueh, Hongli Gong, Liang Zhou","doi":"10.1099/mgen.0.001221","DOIUrl":null,"url":null,"abstract":"<p><p>The aetiological mechanisms of <i>Fusobacterium nucleatum</i> in laryngeal cancer remain unclear. This study aimed to reveal the epigenetic signature induced by <i>F. nucleatum</i> in laryngeal squamous cell carcinoma (LSCC). Combined analysis of methylome and transcriptome data was performed to address the functional role of <i>F. nucleatum</i> in laryngeal cancer. Twenty-nine differentially expressed methylation-driven genes were identified by mapping the methylation levels of significant differential methylation sites to the expression levels of related genes. The combined analysis revealed that <i>F. nucleatum</i> promoted Janus kinase 3 (JAK3) gene expression in LSCC. Further validation found decreased methylation and elevated expression of JAK3 in the <i>F. nucleatum-</i>treated LSCC cell group; <i>F. nucleatum</i> abundance and JAK3 gene expression had a positive correlation in tumour tissues. This analysis provides a novel understanding of the impact of <i>F. nucleatum</i> in the methylome and transcriptome of laryngeal cancer. Identification of these epigenetic regulatory mechanisms opens up new avenues for mechanistic studies to explore novel therapeutic strategies.</p>","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"10 3","pages":""},"PeriodicalIF":4.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10995630/pdf/","citationCount":"0","resultStr":"{\"title\":\"Integrative methylome and transcriptome analysis reveals epigenetic regulation of <i>Fusobacterium nucleatum</i> in laryngeal cancer.\",\"authors\":\"Xiaohui Yuan, Hui-Ching Lau, Huiying Huang, Chi-Yao Hsueh, Hongli Gong, Liang Zhou\",\"doi\":\"10.1099/mgen.0.001221\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aetiological mechanisms of <i>Fusobacterium nucleatum</i> in laryngeal cancer remain unclear. This study aimed to reveal the epigenetic signature induced by <i>F. nucleatum</i> in laryngeal squamous cell carcinoma (LSCC). Combined analysis of methylome and transcriptome data was performed to address the functional role of <i>F. nucleatum</i> in laryngeal cancer. Twenty-nine differentially expressed methylation-driven genes were identified by mapping the methylation levels of significant differential methylation sites to the expression levels of related genes. The combined analysis revealed that <i>F. nucleatum</i> promoted Janus kinase 3 (JAK3) gene expression in LSCC. Further validation found decreased methylation and elevated expression of JAK3 in the <i>F. nucleatum-</i>treated LSCC cell group; <i>F. nucleatum</i> abundance and JAK3 gene expression had a positive correlation in tumour tissues. This analysis provides a novel understanding of the impact of <i>F. nucleatum</i> in the methylome and transcriptome of laryngeal cancer. Identification of these epigenetic regulatory mechanisms opens up new avenues for mechanistic studies to explore novel therapeutic strategies.</p>\",\"PeriodicalId\":18487,\"journal\":{\"name\":\"Microbial Genomics\",\"volume\":\"10 3\",\"pages\":\"\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10995630/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbial Genomics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/mgen.0.001221\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial Genomics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/mgen.0.001221","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Integrative methylome and transcriptome analysis reveals epigenetic regulation of Fusobacterium nucleatum in laryngeal cancer.
The aetiological mechanisms of Fusobacterium nucleatum in laryngeal cancer remain unclear. This study aimed to reveal the epigenetic signature induced by F. nucleatum in laryngeal squamous cell carcinoma (LSCC). Combined analysis of methylome and transcriptome data was performed to address the functional role of F. nucleatum in laryngeal cancer. Twenty-nine differentially expressed methylation-driven genes were identified by mapping the methylation levels of significant differential methylation sites to the expression levels of related genes. The combined analysis revealed that F. nucleatum promoted Janus kinase 3 (JAK3) gene expression in LSCC. Further validation found decreased methylation and elevated expression of JAK3 in the F. nucleatum-treated LSCC cell group; F. nucleatum abundance and JAK3 gene expression had a positive correlation in tumour tissues. This analysis provides a novel understanding of the impact of F. nucleatum in the methylome and transcriptome of laryngeal cancer. Identification of these epigenetic regulatory mechanisms opens up new avenues for mechanistic studies to explore novel therapeutic strategies.
期刊介绍：
Microbial Genomics (MGen) is a fully open access, mandatory open data and peer-reviewed journal publishing high-profile original research on archaea, bacteria, microbial eukaryotes and viruses.
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