利用三维体内肿瘤模型对骨肉瘤细胞系和患者组织进行分析-- Punicalagin 的可能作用

Organoids Pub Date : 2024-03-04 DOI:10.3390/organoids3010004
Anna Rebecca Dorn, Sara Neff, Sophia Hupp, Melissa Engelhardt, Eric Pion, Ulrich Lenze, Carolin Knebel, Anna Duprée, Simone Schewe, Markus Weber, Christian Wulbrand, Axel Hillmann, Florian Weber, Phillip Clarke, Philipp Kainz, Thiha Aung, Silke Haerteis
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引用次数: 0

摘要

骨肉瘤是最常见的原发性恶性骨肿瘤,多发于儿童、青少年和年轻人。尽管目前有手术和多化疗等治疗方法,但转移性骨肉瘤患者的生存率仍然很低。在最近的研究中,Punicalagin 在细胞培养试验中降低了细胞活力、血管生成和侵袭。本研究的目的是在三维体内肿瘤模型中考察潘立卡金对骨肉瘤的影响。人骨肉瘤活检组织、SaOs-2 和 MG-63 细胞在三维体内绒毛膜(CAM)模型中生长。经过长达 72 小时的培养后,肿瘤每天接受 4 天的潘立卡金治疗。对CAM肿瘤进行重量测量,并使用激光斑点对比成像(LSCI)和基于深度学习的图像分析软件(CAM Assay Application v.3.1.0)测量血管生成。移植后进行 HE、Ki-67 和 Caspase-3 染色。骨肉瘤细胞系SaOs-2和MG-63以及骨肉瘤患者组织在CAM上显示出令人满意的生长模式。用 punicalagin 治疗可减少肿瘤重量、增殖和肿瘤诱导的血管生成,而且肿瘤组织显示出促凋亡特性。这些结果为开展进一步研究奠定了坚实的基础,并表明潘立卡金为骨肉瘤患者提供了一种前景广阔的辅助治疗方案。三维体内肿瘤模型是测试抗癌疗法的有益模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of Osteosarcoma Cell Lines and Patient Tissue Using a 3D In Vivo Tumor Model—Possible Effects of Punicalagin
Osteosarcomas are the most common primary malignant bone tumors and mostly affect children, adolescents, and young adults. Despite current treatment options such as surgery and polychemotherapy, the survival of patients with metastatic disease remains poor. In recent studies, punicalagin has reduced the cell viability, angiogenesis, and invasion in cell culture trials. The aim of this study was to examine the effects of punicalagin on osteosarcomas in a 3D in vivo tumor model. Human osteosarcoma biopsies and SaOs-2 and MG-63 cells, were grown in a 3D in vivo chorioallantoic membrane (CAM) model. After a cultivation period of up to 72 h, the tumors received daily treatment with punicalagin for 4 days. Weight measurements of the CAM tumors were performed, and laser speckle contrast imaging (LSCI) and a deep learning-based image analysis software (CAM Assay Application v.3.1.0) were used to measure angiogenesis. HE, Ki-67, and Caspase-3 staining was performed after explantation. The osteosarcoma cell lines SaOs-2 and MG-63 and osteosarcoma patient tissue displayed satisfactory growth patterns on the CAM. Treatment with punicalagin decreased tumor weight, proliferation, and tumor-induced angiogenesis, and the tumor tissue showed pro-apoptotic characteristics. These results provide a robust foundation for the implementation of further studies and show that punicalagin offers a promising supplementary treatment option for osteosarcoma patients. The 3D in vivo tumor model represents a beneficial model for the testing of anti-cancer therapies.
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