在开发具有高抗氧化活性指标的黑茶和绿茶提取物生产技术中选择最佳提取模式

D. Ignatova, Marianna Sergeevna Voronina, N. V. Makarova
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引用次数: 0

摘要

众所周知,萃取过程中的各种因素,如溶剂类型、温度、时间、溶剂系统、原料研磨程度、萃取模数等,都会对植物产品的抗氧化性水平产生重大影响。我们研究的目的是为两种茶叶(山茶)选择最佳萃取模块,以获得总可溶性固形物、酚类、黄酮类、单宁类、DPPH 法抗自由基活性、FRAP 法恢复强度值高的提取物。研究对象是两种茶叶的水提取物,研磨度为 0.5 毫米,提取温度为 40-50°C,提取时间为 24 小时,提取模数为 1:10、1:15、1:20。 当使用提取模数 1:10 时,红茶和绿茶中的干可溶性物质含量指标达到较高值(分别为 9% 和 9.6%),萃取模式为 1 : 10 时,红茶和绿茶提取物中的酚类物质(3139 毫克(HA)/100 克和 2045 毫克(HA)/100 克)、黄酮类物质(1644 毫克(K)/100 克和 718 毫克(K)/100 克)、单宁酸(123 毫克儿茶素/100 克和 56 毫克儿茶素/100 克)的含量最高。萃取模数为 1 : 10 的红茶和绿茶提取物的复原力指数最高(8.73 mmol Fe2+ / kg 和 15.84 mmol Fe2+ / kg)。萃取模数为 1 : 10 时,红茶和绿茶提取物的抗自由基活性最低(Ec50 = 0.39 毫克/立方厘米和 Ec50 = 0.69 毫克/立方厘米)。因此,我们建议将模数 1 : 10 的值作为从红茶和绿茶中提取具有高水平研究参数的提取物的最可接受值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SELECTION OF THE OPTIMAL EXTRACTION MODULE IN THE DEVELOPMENT OF THE TECHNOLOGY OF PRODUCTION OF BLACK AND GREEN TEA EXTRACTS (Camellia sinensis) WITH HIGH INDICATORS OF ANTIOXIDANT ACTIVITY
It is known that various factors of extraction, such as the type of solvent, temperature, time, solvent system, degree of grinding of raw materials, extraction modulus significantly affect the level of antioxidant properties of plant products. The aim of our study was to select the optimal extraction module for two types of tea (Camellia sinensis), to obtain extracts with high values of total soluble solids, phenols, flavonoids, tannins, antiradical activity according to the DPPH method, restoring strength according to the FRAP method. The objects of the study were extracts of two types of tea obtained using water, with a grinding degree of 0.5 mm, an extraction temperature of 40–50 °C, an extraction time of 24 h, and various versions of extraction modules 1 : 10, 1 : 15, 1 : 20. The indicator of the content of dry soluble substances in black and green tea reaches high values when using the extraction module 1 : 10 (9% and 9.6%, respectively), the maximum amount of phenolic substances (3139 mg (HA) / 100 g and 2045 mg (HA) / 100 g), flavonoids (1644 mg (K) / 100 g and 718 mg (K) / 100 g), tannins (123 mg catechin / 100 g and 56 mg catechin / 100 g) were extracted from black and green tea extracts at extraction module 1 : 10. The highest index of restorative power was observed in black and green tea extracts with an extraction modulus of 1 : 10 (8.73 mmol Fe2+ / kg and 15.84 mmol Fe2+ / kg). At an extraction modulus of 1 : 10, black and green tea extracts exhibited the least antiradical activity (Ec50 = 0.39 mg / cm3 and Ec50 = 0.69 mg / cm3). Thus, we can recommend the value of the module 1 : 10 as the most acceptable for obtaining extracts from black and green tea with a high level of the studied parameters.
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