鉴定四种番茄(Lycopersicon esculentum)上的食源性病原体

C. Opara, K. A. Okoronkwo
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引用次数: 0

摘要

番茄(Lycopersicon esculentum)含有大量水分,因此更容易受到食源性病原体(尤其是细菌和真菌)的侵害。这些病原体会产生霉菌毒素,危害人类健康。因此,本研究对尼日利亚巴耶尔萨州斯瓦利市场销售的西红柿进行了食源性病原体鉴定。采用倒平板法在马铃薯葡萄糖琼脂中培养后,从番茄中分离出的病原体有大肠杆菌、志贺氏菌、沙门氏菌、肠杆菌、变形杆菌、棒状杆菌、葡萄球菌、假单胞菌、镰刀菌、乳酸杆菌、弧菌、链霉菌、蜡样芽孢杆菌和梭状芽孢杆菌。宏观和微观检查用于鉴定从番茄中分离出的真菌的形态。从番茄中分离出的真菌有:Vericolor、flavus、Emericella rugulosis、Rhizopus spp、Aspergillus phoreolina、Micropholina phaseolina、Rhizopus solarin、Penecillum oxalina 和霉菌。从所有市场地点分离出的菌株比例分别为:黄曲霉 33.33%、葡萄孢 33.33%、小蚜 33%、皱缩菌 67%和根瘤菌 13.33%。黄曲霉的腐烂直径为 18 毫米,小孢子菌的腐烂直径为 15 毫米,而根瘤菌的腐烂直径为 11 毫米。因此,必须采用适当的处理方法和充足的储存设施来延长番茄的保质期。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of Foodborne Pathogens on Four Species of Tomatoes (Lycopersicon esculentum)
Tomato (Lycopersicon esculentum) contains large amounts of water which makes it more susceptible to foodborne pathogens especially (bacteria and fungi). These pathogens produce mycotoxins that are detrimental to human health. This study was therefore carried out to identify foodborne pathogens, of tomatoes sold in Swali market, Bayelsa state, Nigeria. The isolates from the tomatoes after culturing with Potato Dextrose Agar using pour plate method are: Escherichia coli, Shigella, Salmonella, Enterobacter, Proteus, Corynabacter, Staphylococcus, Pseudomonas, Fusobacterium, Lactobacillus, Vibrio, Streptomyces, Bacillus cereus and Clostridium. While the macroscopic and microscopic examinations were used to identify the morphologies of the fungi isolated from the tomatoes. The following were isolated; Vericolor, flavus, Emericella rugulosis, Rhizopus spp, Aspergillus phoreolina, Micropholina phaseolina, Rhizopus solarin, Penecillum oxalina and mould. The percentage occurrence of the isolates from all the markets locations were; flavus 33.33%, vugulosin 33.33%, Microphoslina 33%, rugulosis 67% and Rhizopus spp 13.33%. The decay diameter of flavus is 18mm, the decay diameter of Microphoslina is 15mm, while the decay diameter of Rhizopus spp is 11mm. Proper handling and adequate storage facilities must therefore be employed to prolong the shelf life of tomatoes.
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