胸腺醌对前列腺癌脂滴形成相关的 PLA2G7、UCP2 和 NEDD4L 基因蛋白水平的影响

Online Türk Sağlık Bilimleri Dergisi Pub Date : 2024-03-11 DOI:10.26453/otjhs.1422576

Abdelhak Hadjmi̇moune, Ahmet Çarhan, Beyza Ecem Öz Bedir, Humeyra Yilmaz, M. Erçin, Ender Şimşek

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引用次数: 0

摘要

目的：前列腺癌（PCa）患者在接受标准治疗时，除了对阉割产生抗药性外，还会遭受严重的副作用。PCa 细胞的脂肪生成增加。胸腺醌（TQ）可抑制细胞增殖、转移和侵袭。然而，目前还没有关于胸腺醌对 NEDDL4、PLA2G7 和 UCP2 脂滴（LD）相关蛋白水平影响的研究。因此，本研究旨在探讨 TQ 对 DU145 和 PC3 细胞系中 PLA2G7、UCP2 和 NEDD4L 蛋白的影响。材料与方法：培养细胞并用 TQ 处理，DU145 和 PC3 的 IC50 分别为 60 µM 和 80 µM。用酶联免疫吸附法测定 PLA2G7、UCP2 和 NEDD4L 的水平。结果显示TQ 能明显提高 NEDD4L 的水平（p

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The Effect of Thymoquinone on the Protein Levels of PLA2G7, UCP2, and NEDD4L Genes Associated with Lipid Droplets Formation in Prostate Cancer

Objective: Prostate cancer (PCa) patients suffer severe side effects of standard treatment beside the resistance to castration. PCa cells shows increased lipogenesis. Thymoquinone (TQ) inhibits cell proliferation, metastasis, and invasion. However, there was no study on the effect of TQ on the levels of NEDDL4, PLA2G7, and UCP2 lipid droplets (LD) related proteins. Hence, the study aims to investigate the impact of TQ on PLA2G7, UCP2, and NEDD4L proteins on DU145 and PC3 cell lines. Materials and Methods: Cells were cultured and treated with TQ with a IC50 of 60 µM and 80 µM for DU145 and PC3, respectively. PLA2G7, UCP2, and NEDD4L levels were measured using the ELISA. Results: TQ has significantly increased the level of NEDD4L (p

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