TAK242 对慢性阻塞性肺病大鼠 MCP-1 和 TGF-β 的影响

Ruicheng Deng, Mingyu Duan, Xiaoyong Ma, Juanxia Chen, Huifang Zhang, Meifang Liu, Chen Jian, Chen Lijun
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引用次数: 0

摘要

目的研究 TAK242 对慢性阻塞性肺病大鼠 MCP-1 和 TGF-β 的调节机制。方法:将 36 只 SD 大鼠随机分为正常组、COPD 对照组和 TAK242 组:将 36 只 SD 大鼠随机分为正常组、COPD 对照组和 TAK242 组。正常组自由采食,其他各组采用熏蒸加脂多糖气管滴注法建立 COPD 实验动物模型。给药后检查肺功能,光镜下苏木精-伊红染色观察肺组织病理变化,q-PCR检测MCP-1和TGF-β的mRNA表达,Western blot和IHC检测大鼠肺组织中MCP-1和TGF-β的蛋白表达。结果与正常组相比,慢性阻塞性肺病对照组大鼠表现出慢性阻塞性肺病的症状和体征,肺功能下降,MCP-1和TGF-β表达增加。与慢性阻塞性肺病对照组相比,TAK242组的MCP-1和TGF-β表达量有所下降。结论MCP-1和TGF-β在慢性阻塞性肺病纤维化的早期阶段起着至关重要的作用。TAK242 可改善 COPD 模型大鼠的气道炎症并抑制其 COPD 肺纤维化的进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of TAK242 on MCP-1 and TGF-β in COPD Rats
Objective: To investigate the mechanism of MCP-1 and TGF-β regulation by TAK242 in COPD rats. Methods: Thirty-six SD rats were randomly divided into normal, COPD control, and TAK242 groups. The normal group was freely fed, and the other groups used the method of fumigation plus lipopolysaccharide tracheal drip to establish an experimental animal model of COPD. After successful modeling, each experimental group received 0.9% NaCl solution and corresponding drugs by intraperitoneal injection for 7 d. After drug administration, lung function was examined; pathological changes in lung tissue were observed by light microscopy with hematoxylin-eosin staining; mRNA expression of MCP-1 and TGF-β was detected by q-PCR; and protein expression of MCP-1 and TGF-β in lung tissue was detected by Western blot and IHC, TGF-β protein expression in rat lung tissue. Results: Compared with the normal group, rats in the COPD control group showed signs and symptoms of COPD, decreased lung function, and increased expression of MCP-1 and TGF-β. The TAK242 group showed decreased expression of MCP-1 and TGF-β compared to the COPD control group. Conclusion: MCP-1, and TGF-β played a crucial role in the early stage of COPD fibrosis. TAK242 could ameliorate airway inflammation and inhibit the progression of COPD lung fibrosis in pre-existing rats in COPD model rats.
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