绿唇贻贝(Perna canaliculus)提取物的体外免疫调节作用研究

Roberta Cardim Lessa, Belgheis Ebrahimi, Hui Li, Xiao Guan, Yan Li, Jun Lu
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引用次数: 0

摘要

免疫系统在抵御外来入侵者方面发挥着至关重要的作用,而各种多不饱和脂肪酸(如α-亚麻酸(ALA)、二十碳五烯酸(EPA)和二十二碳六烯酸(DHA))的平衡会影响免疫细胞功能和整体免疫反应。本研究旨在通过分析贻贝油提取物对免疫细胞系和细胞因子表达的影响,评估它们在调节炎症反应方面的功效。研究人员采用两种萃取方法(有机溶剂萃取和超临界二氧化碳萃取)从两种组织来源(新鲜贻贝和商业贻贝)中提取了四种不同的贻贝油提取物。然后在 T 淋巴细胞(Jurkat)、单核细胞和巨噬细胞(THP-1 和 U-937)上测试了不同浓度的这些提取物。使用 ELISA 对细胞因子水平进行量化。结果表明,溶剂萃取样品对 THP-1 和 U-937 细胞中肿瘤坏死因子α(TNF-α)和白细胞介素-1β(IL-1β)细胞因子的分泌有剂量依赖性影响,其中商业贻贝粉的提取物比新鲜粉末的提取物更有效。然而,尽管超临界二氧化碳样品中欧米加-3含量较高,但其细胞因子分泌水平却有所升高。此外,100 微克/毫升的新鲜粉末提取物成功地减少了白细胞介素-2(IL-2)的分泌,同时保持了刺激后细胞的活力。研究表明,溶剂萃取的贻贝油能有效调节细胞因子的分泌,调节免疫细胞的活化,缓解炎症。这些发现为利用贻贝油提取物治疗炎症性疾病和增强免疫反应提供了宝贵的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of the In Vitro Immunomodulatory Effects of Extracts from Green-Lipped Mussels (Perna canaliculus)
The immune system plays a crucial role in defending the body against foreign invaders, and the balance of various polyunsaturated fatty acids, such as alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), can impact immune cell functions and overall immune responses. This study aimed to assess the effectiveness of mussel oil extracts in modulating inflammatory responses by analysing their effects on immune cell lines and cytokine expression. Four different mussel oil extracts were obtained using two extraction methods (organic solvent and supercritical CO2 extraction) from two tissue sources (fresh and commercial). These extracts were then tested at various concentrations on T lymphocyte (Jurkat) cells, monocytes, and macrophages (THP-1 and U-937). Cytokine levels were quantified using ELISA. The results showed that the solvent-extracted samples had a dose-dependent effect on tumour necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β) cytokine secretion in THP-1 and U937 cells, with the extract from a commercial mussel powder being more efficient than the extract from fresh powder. However, supercritical CO2 samples showed elevated cytokine secretion levels despite their high omega-3 content. Furthermore, 100 ug/mL extract from fresh powder successfully reduced interleukin-2 (IL-2) secretion while maintaining cell viability after stimulation. The study demonstrated that solvent-extracted mussel oil can effectively regulate cytokine secretion, modulate immune cell activation, and alleviate inflammation. These findings offer valuable insights into using mussel oil extracts to treat inflammatory disorders and enhance immune responses.
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