Kunlathida Luangpraditkun, Preeyanuch Pimjuk, Preeyawass Phimnuan, Wisanee Wisanwattana, Chothip Wisespongpand, N. Waranuch, J. Viyoch
{"title":"大麻叶提取物抗 UVA 辐射的抗衰老特性","authors":"Kunlathida Luangpraditkun, Preeyanuch Pimjuk, Preeyawass Phimnuan, Wisanee Wisanwattana, Chothip Wisespongpand, N. Waranuch, J. Viyoch","doi":"10.3390/cosmetics11020045","DOIUrl":null,"url":null,"abstract":"Hemp extract has garnered interest as a potential cosmeceutical agent with multifunctional activities, particularly in protecting against UV-induced skin cell aberrations and restoring aged skin cells. The ethanolic extract of Cannabis sativa leaves was prepared into an aqueous solution (CLES) to investigate its anti-photoaging ability. HPLC analysis revealed that the CLES contained 1.64 ± 0.01% w/w of cannabidiol and 0.11% w/w of ∆9-tetrahydrocannabinol. Additionally, the total phenolic content was found to be 4.08 ± 0.30 mg gallic acid equivalent per g of solution using the Folin–Ciocalteu method. The CLES exhibited potent scavenging activity using a DPPH assay, with an EC50 value of 277.9 ± 2.41 μg/mL, comparable to L-ascorbic acid, with 2.19 ± 0.28 μg/mL. The anti-photoaging potential of the CLES was evaluated using UVA-irradiated and in vitro-aged fibroblasts as a model. Pre-treatment with 20 μg/mL CLES for 24 h significantly alleviated the reduction in type I procollagen and suppressed the overproduction of MMP-1 and IL-6 induced by UVA. Moreover, the percentage of senescence-associated β-galactosidase-expressing cells decreased significantly to 11.9 ± 0.5% in the aged cells treated with CLES compared with untreated cells (18.8 ± 3.8%). These results strongly indicate the cosmeceutical potential of the CLES as an effective active agent for the anti-photoaging prevention and/or treatment.","PeriodicalId":10735,"journal":{"name":"Cosmetics","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anti-Aging Properties of Cannabis sativa Leaf Extract against UVA Irradiation\",\"authors\":\"Kunlathida Luangpraditkun, Preeyanuch Pimjuk, Preeyawass Phimnuan, Wisanee Wisanwattana, Chothip Wisespongpand, N. Waranuch, J. Viyoch\",\"doi\":\"10.3390/cosmetics11020045\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Hemp extract has garnered interest as a potential cosmeceutical agent with multifunctional activities, particularly in protecting against UV-induced skin cell aberrations and restoring aged skin cells. The ethanolic extract of Cannabis sativa leaves was prepared into an aqueous solution (CLES) to investigate its anti-photoaging ability. HPLC analysis revealed that the CLES contained 1.64 ± 0.01% w/w of cannabidiol and 0.11% w/w of ∆9-tetrahydrocannabinol. Additionally, the total phenolic content was found to be 4.08 ± 0.30 mg gallic acid equivalent per g of solution using the Folin–Ciocalteu method. The CLES exhibited potent scavenging activity using a DPPH assay, with an EC50 value of 277.9 ± 2.41 μg/mL, comparable to L-ascorbic acid, with 2.19 ± 0.28 μg/mL. The anti-photoaging potential of the CLES was evaluated using UVA-irradiated and in vitro-aged fibroblasts as a model. Pre-treatment with 20 μg/mL CLES for 24 h significantly alleviated the reduction in type I procollagen and suppressed the overproduction of MMP-1 and IL-6 induced by UVA. Moreover, the percentage of senescence-associated β-galactosidase-expressing cells decreased significantly to 11.9 ± 0.5% in the aged cells treated with CLES compared with untreated cells (18.8 ± 3.8%). These results strongly indicate the cosmeceutical potential of the CLES as an effective active agent for the anti-photoaging prevention and/or treatment.\",\"PeriodicalId\":10735,\"journal\":{\"name\":\"Cosmetics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-03-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cosmetics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/cosmetics11020045\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cosmetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/cosmetics11020045","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Anti-Aging Properties of Cannabis sativa Leaf Extract against UVA Irradiation
Hemp extract has garnered interest as a potential cosmeceutical agent with multifunctional activities, particularly in protecting against UV-induced skin cell aberrations and restoring aged skin cells. The ethanolic extract of Cannabis sativa leaves was prepared into an aqueous solution (CLES) to investigate its anti-photoaging ability. HPLC analysis revealed that the CLES contained 1.64 ± 0.01% w/w of cannabidiol and 0.11% w/w of ∆9-tetrahydrocannabinol. Additionally, the total phenolic content was found to be 4.08 ± 0.30 mg gallic acid equivalent per g of solution using the Folin–Ciocalteu method. The CLES exhibited potent scavenging activity using a DPPH assay, with an EC50 value of 277.9 ± 2.41 μg/mL, comparable to L-ascorbic acid, with 2.19 ± 0.28 μg/mL. The anti-photoaging potential of the CLES was evaluated using UVA-irradiated and in vitro-aged fibroblasts as a model. Pre-treatment with 20 μg/mL CLES for 24 h significantly alleviated the reduction in type I procollagen and suppressed the overproduction of MMP-1 and IL-6 induced by UVA. Moreover, the percentage of senescence-associated β-galactosidase-expressing cells decreased significantly to 11.9 ± 0.5% in the aged cells treated with CLES compared with untreated cells (18.8 ± 3.8%). These results strongly indicate the cosmeceutical potential of the CLES as an effective active agent for the anti-photoaging prevention and/or treatment.