利用激光显微镜无标记检测 SARS-CoV-2 病毒的直接病毒捕获分析法

IF 2.1 4区医学 Q3 VIROLOGY

Future Virology Pub Date : 2024-03-18 DOI:10.2139/ssrn.4494421

Alessandro Pennesi, G. Ferrari, Federica Giardina, Sara Piselli, Roberto Lo Savio, Adolfo Carloni, S. Paolucci, F. Baldanti

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引用次数: 0

摘要

目的：评估一种称为直接病毒捕获（DVC）的无标记、不依赖变体的检测方法，利用一种称为纳米眼设备病毒检测器（NED-VD）的光学倒置激光显微镜，通过光散射检测完整的 SARS-CoV-2 病毒。检测方法DVC 检测涉及印在玻璃盖玻片基底上的 ACE2 受体与病毒外表面的 S 蛋白之间的相互作用。研究使用了 191 份人类拭子标本。结果显示与 RT-PCR 检测法相比，DVC 检测法的灵敏度为 40.5%，特异性为 90.4%，准确率为 46%。结论该研究提出了一种很有前景的定性预筛检测方法，可用于评估整个病毒的存在并减少 PCR 检测的次数。

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Direct virus capture assay for label-free detection of SARS-CoV-2 virions using laser microscopy

Aim: To evaluate a label-free and variant-independent assay called direct virus capture (DVC) for detection of intact SARS-CoV-2 virions through light scattering, utilizing an optical inverted laser microscope called nano eye device virus detector (NED-VD). Methods: The DVC assay involves the interaction between ACE2 receptors printed on a glass coverslip substrate, and the S protein on the outer surface of virions. The study was conducted using 191 human swab specimens. Results: In comparison to the RT-PCR assay, the DVC method achieved a sensitivity of 40.5%, specificity of 90.4% and accuracy of 46%. Conclusion: The study presents a promising qualitative pre-screening test to evaluate the presence of whole virions and reduce the number of PCR tests.

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来源期刊

Future Virology 医学-病毒学

CiteScore

4.00

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.