利用计算图像分析确定再活化弓形虫病模型中弓形虫脑囊肿的结构特征

IF 3.6 2区数学 Q1 MATHEMATICS, INTERDISCIPLINARY APPLICATIONS

Fractal and Fractional Pub Date : 2024-03-18 DOI:10.3390/fractalfract8030175

N. Bauman, J. Srbljanović, Ivana Čolović Čalovski, Olivera Lijeskić, V. Ćirković, Jelena Trajković, B. Bobić, Andjelija Ž. Ilić, T. Štajner

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引用次数: 0

摘要

弓形虫（Toxoplasma gondii）是一种必须存在于细胞内的寄生虫，有三个感染生命阶段--速殖体、缓殖体和孢子虫。组织囊肿破裂后，缓虫重新转化为弓形虫，导致免疫力低下的宿主感染再活化弓形虫病（RT）。本研究的目的是应用 ImageJ 软件分析从一种新建立的 RT 体内模型中获得的刚地弓形虫脑囊肿。用环磷酰胺和氢化可的松治疗慢性感染淋病双球菌（BGD1 和 BGD26 株系）的小鼠（实验组-EG），或作为感染对照组（IC）不进行治疗。小鼠的 RT 通过 qPCR（PCR+）确认；仍处于慢性感染的小鼠为 PCR-。共对 90 张囊肿图像进行了分形维度 (FD)、裂隙度 (L)、直径 (D)、圆度 (C) 和堆积密度 (PD) 分析。与 IC 小鼠相比，PCR+ 小鼠的圆度明显更高（BGD1 小鼠 p < 0.05，BGD26 株小鼠 p < 0.001）。仅在 BGD1 品系的 IC 中观察到 D 和 PD 之间存在明显的负相关（ρ = -0.384，p = 0.048），而分形参数则保持稳定。与攻击性更强的 BGD26 株系相比，BGD1 株系的 D、C 和 PD 明显更高，而裂隙度 L 则更低。总之，这些结果表明了弓形虫囊肿在免疫受损宿主体内结构改变的复杂性，并强调了 ImageJ 在弓形虫病实验模型中的应用潜力。

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Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis

Toxoplasma gondii is an obligate intracellular parasite existing in three infectious life stages—tachyzoites, bradyzoites, and sporozoites. Rupture of tissue cysts and re-conversion of bradyzoites to tachyzoites leads to reactivated toxoplasmosis (RT) in an immunocompromised host. The aim of this study was to apply ImageJ software for analysis of T. gondii brain cysts obtained from a newly established in vivo model of RT. Mice chronically infected with T. gondii (BGD1 and BGD26 strains) were treated with cyclophosphamide and hydrocortisone (experimental group—EG) or left untreated as infection controls (ICs). RT in mice was confirmed by qPCR (PCR+); mice remaining chronically infected were PCR−. A total of 90 images of cysts were analyzed for fractal dimension (FD), lacunarity (L), diameter (D), circularity (C), and packing density (PD). Circularity was significantly higher in PCR+ compared to IC mice (p < 0.05 for BGD1, p < 0.001 for the BGD26 strain). A significant negative correlation between D and PD was observed only in IC for the BGD1 strain (ρ = −0.384, p = 0.048), while fractal parameters were stable. Significantly higher D, C, and PD and lower lacunarity, L, were noticed in the BGD1 compared to the more aggressive BGD26 strain. In conclusion, these results demonstrate the complexity of structural alterations of T. gondii cysts in an immunocompromised host and emphasize the application potential of ImageJ in the experimental models of toxoplasmosis.

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来源期刊

Fractal and Fractional MATHEMATICS, INTERDISCIPLINARY APPLICATIONS-

CiteScore

4.60

自引率

18.50%

发文量

632

审稿时长

11 weeks

期刊介绍： Fractal and Fractional is an international, scientific, peer-reviewed, open access journal that focuses on the study of fractals and fractional calculus, as well as their applications across various fields of science and engineering. It is published monthly online by MDPI and offers a cutting-edge platform for research papers, reviews, and short notes in this specialized area. The journal, identified by ISSN 2504-3110, encourages scientists to submit their experimental and theoretical findings in great detail, with no limits on the length of manuscripts to ensure reproducibility. A key objective is to facilitate the publication of detailed research, including experimental procedures and calculations. "Fractal and Fractional" also stands out for its unique offerings: it warmly welcomes manuscripts related to research proposals and innovative ideas, and allows for the deposition of electronic files containing detailed calculations and experimental protocols as supplementary material.