恩达利治疗可改善镰状细胞相关的肠屏障功能紊乱,并延长镰状细胞小鼠的存活时间。

IF 2 4区 医学 Q3 HEMATOLOGY
Hematology Pub Date : 2024-12-01 Epub Date: 2024-03-25 DOI:10.1080/16078454.2024.2331940
Elio Haroun, Seah H Lim, Dibyendu Dutta
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引用次数: 0

摘要

背景:Endari(L-谷氨酰胺)是一种条件性氨基酸,可降低镰状细胞病(SCD)血管闭塞危象(VOC)的发生频率:我们用 Endari 治疗雌性 Townes SCD 小鼠,并通过测定血清中口服的异硫氰酸荧光素(FITC)共轭葡聚糖 4 kDa 的恢复情况,以及通过 ELISA 测定血清中肠脂肪酸结合蛋白(iFABP)和脂多糖(LPS)的浓度来评估它们的肠屏障功能。我们还探讨了 Endari 对反复实验诱发 VOC 的 SCD 小鼠存活率的影响:结果:与只接受水治疗的SCD小鼠相比,接受恩达利治疗的小鼠的肠道屏障功能有所改善,屏障通透性降低,脂多糖从肠腔进入血液循环的转运量减少。这些变化仅在 Endari 治疗 4 周后发生。肠道屏障功能的改善还与恩达利治疗的 SCD 小鼠在反复实验诱导的 VOC 后存活时间的延长有关:我们的研究结果为恩达立改善肠道屏障功能及相关的 SCD 存活率提供了证据支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Endari treatment ameliorates sickle cell-related disruption in intestinal barrier functions and is associated with prolonged survival in sickle cell mice.

Background: Endari (L-glutamine) is a conditional amino acid that reduces the frequency of vaso-occlusive crisis (VOC) in sickle cell disease (SCD).

Aim: To investigate whether Endari could ameliorate intestinal barrier function and improve survival outcomes in SCD.

Methods: We treated female Townes SCD mice with Endari and evaluated their intestinal barrier functions by measuring the recovery of orally administered fluorescein isothiocyanate (FITC)-conjugated dextran 4 kDa in serum, and serum intestinal fatty acid binding proteins (iFABP) and lipopolysaccharide (LPS) concentrations by ELISA. We also explored the impact the Endari has on the survival of the SCD mice that underwent repeated experimentally-induced VOC.

Results: Compared to SCD mice treated with water only, Endari-treated mice showed improved intestinal barrier functions, with decrease in the barrier permeability and reduction in the translocation of lipopolysaccharides from the intestinal lumen into the circulation. These changes occurred after only 4 weeks of Endari treatment. Improved intestinal barrier function was also associated with prolonged survival in Endari-treated SCD mice after repeated experimentally-induced VOC.

Conclusion: Our findings provide the evidence supporting the beneficial effects of Enadri in improving intestinal barrier function and associated survival outcomes in SCD.

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来源期刊
Hematology
Hematology 医学-血液学
CiteScore
2.60
自引率
5.30%
发文量
140
审稿时长
3 months
期刊介绍: Hematology is an international journal publishing original and review articles in the field of general hematology, including oncology, pathology, biology, clinical research and epidemiology. Of the fixed sections, annotations are accepted on any general or scientific field: technical annotations covering current laboratory practice in general hematology, blood transfusion and clinical trials, and current clinical practice reviews the consensus driven areas of care and management.
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