水溶性柯罗纳汀对在植物细胞培养物中用不溶于水的 1-丁基-1-甲基吡咯烷鎓双(三氟甲磺酰)亚胺生产紫杉醇的影响

Waka NISHIKAWA, Momoka TABATA, Yuka IZAKI, Shinjiro YAMAMOTO, Shuhei HAYASHI, Hitoshi MIYASAKA
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引用次数: 0

摘要

我们在此报告了水溶性诱导剂冕宁对植物细胞培养物中紫杉醇产生量的影响,该培养物是用不溶于水的离子液体--1-丁基-1-甲基吡咯烷鎓双(三氟甲磺酰)亚胺(P14TFSI)原位萃取的。不含 P14TFSI 的细胞培养产生的紫杉醇量随冠脉宁浓度的增加而增加,但由于所产生的紫杉醇的反馈抑制作用,含冠脉宁的细胞生长量低于对照组。加入 P14TFSI 和冠脉宁的培养物的细胞生长量比对照组的细胞生长量增加得多,这是因为 P14TFSI 原位提取减少了产生的紫杉醇。两相培养中紫杉醇的产生量随冠脉宁浓度的增加而增加,在冠脉宁浓度为 1 µM 时达到峰值,然后下降。在含有P14TFSI和1 µM冕宁的培养物中,紫杉醇的产量是对照培养物的250倍,这可能是由于冕宁和P14TFSI的非生物胁迫的协同作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Water-Soluble Coronatine on Production of Paclitaxel in a Plant Cell Culture with Water-immiscible 1-Butyl-1-methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

We report here the effect of a water-soluble elicitor, coronatine, on the amount of paclitaxel produced in a plant cell culture by in situ extraction with a water-immiscible ionic liquid, 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14TFSI). The amount of paclitaxel produced in the cell culture without P14TFSI enhanced with increase of coronatine concentration, however, the amount of cell growth including coronatine was lower than that of the control because of feedback inhibition by the produced paclitaxel. The amount of the cell growth in the culture with P14TFSI and coronatine increased more than that in the control culture because the produced paclitaxel was decreased by in situ extraction with P14TFSI. The production amount of paclitaxel in the two-phase culture increased with increase of coronatine concentration, reached the peak in that including 1 µM coronatine and decreased. The production amount of paclitaxel in the culture including P14TFSI and 1 µM coronatine was 250 times greater than that of the control culture, possibly due to the synergistic effects of coronatine and abiotic stress of P14TFSI.

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