利用液相色谱-串联质谱法开发分析炒米中麦角内酯毒素的简易筛选方法。

IF 2.8 4区 医学 Q2 TOXICOLOGY
Forensic Toxicology Pub Date : 2024-07-01 Epub Date: 2024-03-22 DOI:10.1007/s11419-024-00683-3
Hiroshi Koike, Maki Kanda, Chie Monma, Souichi Yoshikawa, Hiroshi Hayashi, Yoko Matsushima, Yumi Ohba, Momoka Hayashi, Natsumi Furuta, Wakaba Okada, Chieko Nagano, Keiko Yokoyama, Tomoko Yokoyama, Takeo Sasamoto
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引用次数: 0

摘要

目的:即使在无法检测到蜡样芽孢杆菌的情况下,炒饭样本中存在蜡样芽孢杆菌内酯(一种由蜡样芽孢杆菌产生的催吐毒素)也是食物中毒的重要证据。本研究旨在利用 QuEChERS 程序和液相色谱-串联质谱法(LC-MS/MS),开发一种分析炒饭中蜡样内酯的筛选方法:方法:采用 QuEChERS 萃取法和液相色谱-串联质谱法对炒米样品中的谷维素进行鉴定和定量。结果:QuEChERS 方法去除了样品中的基质(10 微克/千克和 50 微克/千克),而 LC-MS/MS 方法去除了样品中的基质(10 微克/千克和 50 微克/千克),而 LC-MS/MS 方法去除了样品中的基质(50 微克/千克):结果:QuEChERS 程序可去除炒米中的基质化合物。通过特征性的 MS/MS 图谱可以鉴定出麦角苷。由于 7 个炒米样品中的基质效应在 ± 6% 以内,因此可以使用外部溶剂校准曲线进行定量。该方法的准确度为88%至89%。重复性和实验室内重现性的相对标准偏差均为结论:本文所述的 QuEChERS 提取程序作为一种可靠的筛查炒米样品中谷胱甘肽的工具,显示出了巨大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Development of a simple screening method for analyzing cereulide toxin in fried rice using liquid chromatography-tandem mass spectrometry.

Development of a simple screening method for analyzing cereulide toxin in fried rice using liquid chromatography-tandem mass spectrometry.

Purpose: The presence of cereulide, an emetic toxin produced by Bacillus cereus, in fried rice samples is critical evidence of food poisoning even in situations where B. cereus could not be detected. This study aims to develop a screening method for analyzing cereulide in fried rice using the QuEChERS procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Methods: Cereulide was identified and quantified in fried rice samples using the QuEChERS extraction method and LC-MS/MS. The accuracies of the methods were determined by analyzing fortified blank samples at two concentrations (10 and 50 µg/kg) conducted on three samples daily for five days.

Results: The QuEChERS procedure removed matrix compounds from fried rice. Characteristic MS/MS spectra enabled the identification of cereulide. As the matrix effects in seven fried rice samples were within ± 6%, an external solvent calibration curve could be used for quantification. This method exhibited good accuracy ranging from 88 to 89%. The relative standard deviations for both repeatability and intra-laboratory reproducibility were < 4%. These standard deviations satisfied the criteria of the Japanese validation guidelines for residues (MHLW 2010, Director Notice, Syoku-An No. 1224-1). The limit of quantification was 2 μg/kg. The applicability of this method was confirmed using the analysis of cereulide in fried rice samples incubated with emetic Bacillus cereus.

Conclusions: The QuEChERS extraction procedure described herein showed substantial promise as a reliable screening tool for cereulide in fried rice sample.

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来源期刊
Forensic Toxicology
Forensic Toxicology TOXICOLOGY-
CiteScore
5.80
自引率
9.10%
发文量
40
审稿时长
3 months
期刊介绍: The journal Forensic Toxicology provides an international forum for publication of studies on toxic substances, drugs of abuse, doping agents, chemical warfare agents, and their metabolisms and analyses, which are related to laws and ethics. It includes original articles, reviews, mini-reviews, short communications, and case reports. Although a major focus of the journal is on the development or improvement of analytical methods for the above-mentioned chemicals in human matrices, appropriate studies with animal experiments are also published. Forensic Toxicology is the official publication of the Japanese Association of Forensic Toxicology (JAFT) and is the continuation of the Japanese Journal of Forensic Toxicology (ISSN 0915-9606).
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