内共生菌 Tremblaya phenacola 通过调节 mTOR 途径影响棉花蚧的繁殖。

IF 10.8 1区 环境科学与生态学 Q1 ECOLOGY
Jianyang Bai, Zhangqi Zuo, Haonan DuanMu, Meizhen Li, Haojie Tong, Yang Mei, Yiqi Xiao, Kang He, Mingxing Jiang, Shuping Wang, Fei Li
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引用次数: 0

摘要

内共生关系错综复杂的进化动态导致共生体基因组具有独特的特征,从而对宿主昆虫的表型产生深远影响。在这里,我们研究了 Phenacoccinae 亚科臭名昭著的害虫 Phenacoccus solenopsis 的内共生系统。内共生菌 "Candidatus Tremblaya phenacola"(T. phenacola PSOL)在雌性宿主的整个生命周期中持续存在,在产卵期间更为活跃,而在雄性宿主化蛹后数量显著下降。基因组测序得出的内共生体基因组大小为 221.1 kb,由 7 个等位基因组成,源于 betaproteobacteria 和 gammaproteobacteria 之间的嵌合排列。对氨基酸代谢途径的全面分析表明,宿主和内共生体的代谢具有互补性。通过抗生素处理消除 T. phenacola PSOL 能显著降低拟南芥的繁殖力。加权基因共表达网络分析(WGCNA)表明,与必需氨基酸合成相关的基因与宿主减数分裂和卵母细胞成熟相关的基因之间存在相关性。此外,改变内源共生体的丰度会激活宿主的 mTOR 通路,这表明氨基酸丰度的变化会通过这一信号通路影响宿主的生殖能力。总之,这些发现证明了内共生菌 T. phenacola PSOL 对拟南芥高繁殖力的贡献机制,并为营养补偿和内共生系统的共同进化提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Endosymbiont Tremblaya phenacola influences the reproduction of cotton mealybugs by regulating the mechanistic target of rapamycin pathway.

The intricate evolutionary dynamics of endosymbiotic relationships result in unique characteristics among the genomes of symbionts, which profoundly influence host insect phenotypes. Here, we investigated an endosymbiotic system in Phenacoccus solenopsis, a notorious pest of the subfamily Phenacoccinae. The endosymbiont, "Candidatus Tremblaya phenacola" (T. phenacola PSOL), persisted throughout the complete life cycle of female hosts and was more active during oviposition, whereas there was a significant decline in abundance after pupation in males. Genome sequencing yielded an endosymbiont genome of 221.1 kb in size, comprising seven contigs and originating from a chimeric arrangement between betaproteobacteria and gammaproteobacteria. A comprehensive analysis of amino acid metabolic pathways demonstrated complementarity between the host and endosymbiont metabolism. Elimination of T. phenacola PSOL through antibiotic treatment significantly decreased P. solenopsis fecundity. Weighted gene coexpression network analysis demonstrated a correlation between genes associated with essential amino acid synthesis and those associated with host meiosis and oocyte maturation. Moreover, altering endosymbiont abundance activated the host mechanistic target of rapamycin pathway, suggesting that changes in the amino acid abundance affected the host reproductive capabilities via this signal pathway. Taken together, these findings demonstrate a mechanism by which the endosymbiont T. phenacola PSOL contributed to high fecundity in P. solenopsis and provide new insights into nutritional compensation and coevolution of the endosymbiotic system.

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来源期刊
ISME Journal
ISME Journal 环境科学-生态学
CiteScore
22.10
自引率
2.70%
发文量
171
审稿时长
2.6 months
期刊介绍: The ISME Journal covers the diverse and integrated areas of microbial ecology. We encourage contributions that represent major advances for the study of microbial ecosystems, communities, and interactions of microorganisms in the environment. Articles in The ISME Journal describe pioneering discoveries of wide appeal that enhance our understanding of functional and mechanistic relationships among microorganisms, their communities, and their habitats.
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