Xuejing Wang , Fangyuan Peng , Shuli Yuan , Zhen Huang , Lingwei Tang , Song Chen , Jinhui Liu , Wen Fu , Liangyue Peng , Wenbin Liu , Yamei Xiao
{"title":"GCN2-eIF2α信号通路对三倍体鲫鱼的生长有负向调节作用。","authors":"Xuejing Wang , Fangyuan Peng , Shuli Yuan , Zhen Huang , Lingwei Tang , Song Chen , Jinhui Liu , Wen Fu , Liangyue Peng , Wenbin Liu , Yamei Xiao","doi":"10.1016/j.ygeno.2024.110832","DOIUrl":null,"url":null,"abstract":"<div><p>GCN2-eIF2α signaling pathway plays crucial roles in cell growth,development, and protein synthesis. However, in polyploid fish, the function of this pathway is rarely understood. In this study, genes associated with the GCN2-eIF2α pathway (<em>pkr, pek, gcn2, eif2α</em>) are founded lower expression levels in the triploid crucian carp (3nCC) muscle compared to that of the red crucian carp (RCC). In muscle effect stage embryos of the 3nCC, the mRNA levels of this pathway genes are generally lower than those of RCC, excluding <em>hri</em> and <em>fgf21.</em> Inhibiting <em>gcn2</em> in 3nCC embryos downregulates downstream gene expression (<em>eif2α, atf4, fgf21</em>), accelerating embryonic development. In contrast, overexpressing of <em>eif2α</em> can alter the expression levels of downstream genes (<em>atf4</em> and <em>fgf21</em>), and decelerates the embryonic development. These results demonstrate the GCN2-eIF2α pathway's regulatory impact on 3nCC growth, advancing understanding of fish rapid growth genetics and offering useful molecular markers for breeding of excellent strains.</p></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"116 3","pages":"Article 110832"},"PeriodicalIF":3.4000,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0888754324000533/pdfft?md5=4a195c211f288171419c13fca25a5579&pid=1-s2.0-S0888754324000533-main.pdf","citationCount":"0","resultStr":"{\"title\":\"GCN2-eIF2α signaling pathway negatively regulates the growth of triploid crucian carp\",\"authors\":\"Xuejing Wang , Fangyuan Peng , Shuli Yuan , Zhen Huang , Lingwei Tang , Song Chen , Jinhui Liu , Wen Fu , Liangyue Peng , Wenbin Liu , Yamei Xiao\",\"doi\":\"10.1016/j.ygeno.2024.110832\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>GCN2-eIF2α signaling pathway plays crucial roles in cell growth,development, and protein synthesis. However, in polyploid fish, the function of this pathway is rarely understood. In this study, genes associated with the GCN2-eIF2α pathway (<em>pkr, pek, gcn2, eif2α</em>) are founded lower expression levels in the triploid crucian carp (3nCC) muscle compared to that of the red crucian carp (RCC). In muscle effect stage embryos of the 3nCC, the mRNA levels of this pathway genes are generally lower than those of RCC, excluding <em>hri</em> and <em>fgf21.</em> Inhibiting <em>gcn2</em> in 3nCC embryos downregulates downstream gene expression (<em>eif2α, atf4, fgf21</em>), accelerating embryonic development. In contrast, overexpressing of <em>eif2α</em> can alter the expression levels of downstream genes (<em>atf4</em> and <em>fgf21</em>), and decelerates the embryonic development. These results demonstrate the GCN2-eIF2α pathway's regulatory impact on 3nCC growth, advancing understanding of fish rapid growth genetics and offering useful molecular markers for breeding of excellent strains.</p></div>\",\"PeriodicalId\":12521,\"journal\":{\"name\":\"Genomics\",\"volume\":\"116 3\",\"pages\":\"Article 110832\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0888754324000533/pdfft?md5=4a195c211f288171419c13fca25a5579&pid=1-s2.0-S0888754324000533-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genomics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0888754324000533\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genomics","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0888754324000533","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
GCN2-eIF2α signaling pathway negatively regulates the growth of triploid crucian carp
GCN2-eIF2α signaling pathway plays crucial roles in cell growth,development, and protein synthesis. However, in polyploid fish, the function of this pathway is rarely understood. In this study, genes associated with the GCN2-eIF2α pathway (pkr, pek, gcn2, eif2α) are founded lower expression levels in the triploid crucian carp (3nCC) muscle compared to that of the red crucian carp (RCC). In muscle effect stage embryos of the 3nCC, the mRNA levels of this pathway genes are generally lower than those of RCC, excluding hri and fgf21. Inhibiting gcn2 in 3nCC embryos downregulates downstream gene expression (eif2α, atf4, fgf21), accelerating embryonic development. In contrast, overexpressing of eif2α can alter the expression levels of downstream genes (atf4 and fgf21), and decelerates the embryonic development. These results demonstrate the GCN2-eIF2α pathway's regulatory impact on 3nCC growth, advancing understanding of fish rapid growth genetics and offering useful molecular markers for breeding of excellent strains.
期刊介绍:
Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation.
As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.