外周血单核细胞的环状 RNA 表达与吸烟慢性阻塞性肺病急性加重的风险有关
IF 2.8
3区 医学
Q1 Medicine
International Journal of Chronic Obstructive Pulmonary Disease
Pub Date : 2024-03-20
DOI:10.2147/copd.s448759
引用次数: 0
摘要
目的：环状 RNA（circRNA）是新发现的内源性非编码 RNA，在多种疾病的发展过程中发挥着重要的基因调节作用。本研究通过评估慢性阻塞性肺病（COPD）患者外周血单核细胞（PBMC）中循环RNA的表达水平，试图为COPD筛查寻找新的生物标志物：我们证实了慢性阻塞性肺病患者（41 人）与对照组（29 人）的 PBMCs 中 circRNA 表达的变化。进一步分析的重点是最高和最低的 circRNA 表达水平。采用 T 检验评估吸烟和非吸烟队列中 COPD 患者的 circRNA 统计差异。此外，在吸烟者中，斯皮尔曼相关性检验评估了 circRNA 与临床指标之间的关联：结果：在 COPD 患者的 PBMCs 中发现并验证了两个高度上调和下调的 circRNA，即 hsa_circ_0042590 和 hsa_circ_0049875。与非吸烟者相比，慢性阻塞性肺病吸烟者的 hsa_circ_0042590 较低，而 hsa_circ_0049875 较高。慢性阻塞性肺病吸烟者上一年和下一年的急性加重（AE）次数之间存在明显的相关性（r=0.52，P< 0.01）（r=0.67，P< 0.001）。此外，hsa_circ_0049875 还与前一年（r=0.68，P< 0.0001）和后一年（r=0.72，P< 0.0001）的 AEs 数量有关。AUC：根据 ROC 曲线分析，hsa_circ_0049875 的 AUC：0.79，95% CI：0.1210- 0.3209，P< 0.0001）对慢性阻塞性肺病有很强的诊断价值。Hsa_circ_0042590 的 AUC 为 0.83，95% CI 为 0.1972--0.0739（P< 0.0001），紧随其后：这项研究发现，吸烟与 COPD PBMCs 中的 hsa_circ_0049875 和 hsa_circ_0042590 密切相关。在吸烟的 COPD 患者中，前几年和随后几年的 AE 数量与 hsa_circ_0042590 和 hsa_circ_0049875 的存在密切相关。此外，根据我们的研究，hsa_circ_0049875和hsa_circ_0042590可能是诊断慢性阻塞性肺疾病的有价值的生物标志物。 关键词：慢性阻塞性肺疾病；环状 RNAs；吸烟；急性加重；生物标志物本文章由计算机程序翻译，如有差异，请以英文原文为准。
Circular RNA Expression of Peripheral Blood Mononuclear Cells Associated with Risk of Acute Exacerbation in Smoking Chronic Obstructive Pulmonary Disease
Purpose: Circular RNAs (circRNAs) are newly identified endogenous non-coding RNAs that function as crucial gene modulators in the development of several diseases. By assessing the expression levels of circRNAs in peripheral blood mononuclear cells (PBMCs) from patients with chronic obstructive pulmonary disease (COPD), this study attempted to find new biomarkers for COPD screening.
Patients and Methods: We confirmed altered circRNA expression in PBMCs of COPD (n=41) vs controls (n=29). Further analysis focused on the highest and lowest circRNA expression levels. The T-test is used to assess the statistical variances in circRNAs among COPD patients in the smoking and non-smoking cohorts. Additionally, among smokers, the Spearman correlation test assesses the association between circRNAs and clinical indicators.
Results: Two circRNAs, hsa_circ_0042590 and hsa_circ_0049875, that were highly upregulated and downregulated in PBMCs from COPD patients were identified and verified. Smokers with COPD had lower hsa_circ_0042590 and higher hsa_circ_0049875, in comparison to non-smokers. There was a significant correlation (r=0.52, P< 0.01) between the number of acute exacerbations (AEs) that smokers with COPD experienced in the previous year and the following year (r=0.67, P< 0.001). Moreover, hsa_circ_0049875 was connected to the quantity of AEs in the year prior (r=0.68, P< 0.0001) as well as the year after (r=0.72, P< 0.0001). AUC: 0.79, 95% CI: 0.1210– 0.3209, P< 0.0001) for hsa_circ_0049875 showed a strong diagnostic value for COPD, according to ROC curve analysis. Hsa_circ_0042590 showed a close second with an AUC of 0.83 and 95% CI: − 0.1972--0.0739 (P < 0.0001).
Conclusion: This research identified a strong correlation between smoking and hsa_circ_0049875 and hsa_circ_0042590 in COPD PBMCs. The number of AEs in the preceding and succeeding years was substantially linked with the existence of hsa_circ_0042590 and hsa_circ_0049875 in COPD patients who smoke. Additionally, according to our research, hsa_circ_0049875 and hsa_circ_0042590 may be valuable biomarkers for COPD diagnosis.
Keywords: chronic obstructive pulmonary disease, circular RNAs, smoking, acute exacerbation, biomarker
Patients and Methods: We confirmed altered circRNA expression in PBMCs of COPD (n=41) vs controls (n=29). Further analysis focused on the highest and lowest circRNA expression levels. The T-test is used to assess the statistical variances in circRNAs among COPD patients in the smoking and non-smoking cohorts. Additionally, among smokers, the Spearman correlation test assesses the association between circRNAs and clinical indicators.
Results: Two circRNAs, hsa_circ_0042590 and hsa_circ_0049875, that were highly upregulated and downregulated in PBMCs from COPD patients were identified and verified. Smokers with COPD had lower hsa_circ_0042590 and higher hsa_circ_0049875, in comparison to non-smokers. There was a significant correlation (r=0.52, P< 0.01) between the number of acute exacerbations (AEs) that smokers with COPD experienced in the previous year and the following year (r=0.67, P< 0.001). Moreover, hsa_circ_0049875 was connected to the quantity of AEs in the year prior (r=0.68, P< 0.0001) as well as the year after (r=0.72, P< 0.0001). AUC: 0.79, 95% CI: 0.1210– 0.3209, P< 0.0001) for hsa_circ_0049875 showed a strong diagnostic value for COPD, according to ROC curve analysis. Hsa_circ_0042590 showed a close second with an AUC of 0.83 and 95% CI: − 0.1972--0.0739 (P < 0.0001).
Conclusion: This research identified a strong correlation between smoking and hsa_circ_0049875 and hsa_circ_0042590 in COPD PBMCs. The number of AEs in the preceding and succeeding years was substantially linked with the existence of hsa_circ_0042590 and hsa_circ_0049875 in COPD patients who smoke. Additionally, according to our research, hsa_circ_0049875 and hsa_circ_0042590 may be valuable biomarkers for COPD diagnosis.
Keywords: chronic obstructive pulmonary disease, circular RNAs, smoking, acute exacerbation, biomarker
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来源期刊
International Journal of Chronic Obstructive Pulmonary Disease
RESPIRATORY SYSTEM-
CiteScore
5.10
自引率
10.70%
发文量
372
审稿时长
16 weeks
期刊介绍：
An international, peer-reviewed journal of therapeutics and pharmacology focusing on concise rapid reporting of clinical studies and reviews in COPD. Special focus will be given to the pathophysiological processes underlying the disease, intervention programs, patient focused education, and self management protocols. This journal is directed at specialists and healthcare professionals
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