伊朗血友病综合治疗中心(ICHCC)转诊的血友病患者中 HTLV-1 病毒的系统发育分析和频率估计

Pub Date : 2024-03-20 DOI:10.3103/s0891416823040109
Tina Taikandi, Navid Momenifar, Ali Namvar, Alireza Azizi Saraji, Mehdi Norouzi, Cobra Razavi Pashabayg
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引用次数: 0

摘要

摘要人类 T 淋巴细胞病毒 1 型(HTLV-1)与恶性疾病的发生有关,尤其是成人 T 细胞白血病/淋巴瘤(ATLL)和骨髓病/热带痉挛性瘫痪(HAM/TSP)。血友病患者有感染血源性疾病的风险,因此防止病毒和其他污染物的传播至关重要。本研究旨在确定转诊至伊朗血友病综合治疗中心(Iranian Comprehensive Hemophilia Care Center,ICHCC)的伊朗血友病患者中 HTLV-1 的流行率,并对结果进行分析。研究共收集了 320 份血友病患者的血液样本,并使用酶联免疫吸附试验(ELISA)进行筛查。使用巢式 PCR 扩增 HTLV-1 的长末端重复序列(LTR)区,确认血清阳性样本。对 LTR 区片段进行扩增、测序,并用 MEGA 7 进行系统发育分析。四份血清学阳性样本中有三份通过 PCR 得到了确诊,HTLV-1 感染率为 0.9%。对感染 HTLV-1 的血友病患者进行的系统进化分析表明,该病毒属于 a 亚型(宇宙型)和 A 亚群(跨洲型)。研究结果表明,血友病患者可能是 HTLV-1 传播的高危人群。此外,对血液和血液制品进行筛查可在防止病毒在流行地区传播方面发挥关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Phylogenetic Analysis and Frequency Estimation of HTLV-1 Virus in Hemophilic Patients Refer to Iranian Comprehensive Hemophilia Care Center (ICHCC)

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Phylogenetic Analysis and Frequency Estimation of HTLV-1 Virus in Hemophilic Patients Refer to Iranian Comprehensive Hemophilia Care Center (ICHCC)

Abstract

Human T-lymphotropic virus type 1 (HTLV-1) is associated with the development of malignant diseases, particularly adult T-cell leukemia/lymphoma (ATLL) and myelopathy/tropical spastic paraparesis (HAM/TSP). Hemophilia patients are at risk of acquiring blood–borne infections, making it critical to prevent the transmission of viral and other contaminants. The objective of this study was to determine the prevalence of HTLV-1 in Iranian hemophilia patients who were referred to the Iranian Comprehensive Hemophilia Care Center (ICHCC) and to analyze the results. A total of 320 blood samples were collected from hemophilia patients and screened using enzyme-linked immunosorbent assay (ELISA). Seropositive samples were confirmed by amplifying the long terminal repeat (LTR) region of HTLV-1 using nested PCR. The LTR region fragment was amplified, sequenced, and analyzed by MEGA 7 for phylogenetic analysis. Three out of four positive serological samples were confirmed using PCR, resulting in an HTLV-1 infection outbreak of 0.9%. Phylogenetic analysis of hemophilia patients infected with HTLV-1 revealed that the virus belongs to subtype a (Cosmopolitan) and subgroup A (Transcontinental). The findings suggest that hemophilia patients may be at high risk for HTLV-1 transmission. Furthermore, screening of blood and blood products can play a critical role in preventing the spread of the virus in endemic areas.

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