兽疫病毒三种不同实验接种途径的比较研究

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Delia Lacasta , María Cuadra , Alex Gómez , Aurora Ortín , Marta Ruiz de Arcaute , Juan José Ramos , Sergio Villanueva-Saz , M. Teresa Tejedor , Héctor Ruiz , Maite Verde , Ramsés Reina , Teresa Navarro , Pablo Quilez , Ana Rodríguez-Largo , Peter Andrew Windsor
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引用次数: 0

摘要

在九只羔羊身上研究了诱发传染性外皮瘤病的orf病毒(ORFV)实验感染的三种不同方法:皮内接种、皮下注射和上皮划痕。目的是找出最合适的实验方法,使所有受感染的动物都能再现严重程度相似的病变。皮下注射未能在三只受感染的动物中的两只再现orf病变,而分别通过皮内和瘢痕接种的两组动物在12 dpi时的病变数量都明显高于皮下注射组。然而,瘢痕接种后病变从接种部位扩散,在粘膜或皮肤的其他部位没有发现与 ORFV 相关的病变。最后,皮内接种后,所有受感染动物的 ORFV 相关病变均呈均匀发展,病变从接种点开始在嘴唇皮肤的不同区域发展,但也会扩散到口腔内部、牙龈、上颚和舌头,就像自然感染时发生的情况一样。因此,得出的结论是,对于研究治疗和疫苗接种新方法的有效性,以改善受影响动物的福利和控制 ORFV 传播的研究而言,实验性 ORFV 感染的最合适途径是皮内接种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative study of three different routes of experimental inoculation of the orf virus

Three different methods of inducing experimental infection of lambs with orf virus (ORFV), the cause of Contagious Ecthyma, were examined in nine animals; intradermal inoculation, subcutaneous injection and epithelial scarification. The objective was to identify the most appropriate experimental method to reproduce the disease with lesions of similar severity in all infected animals. Subcutaneous inoculation failed to reproduce orf lesions in two of the three infected animals, whereas both the groups that were inoculated by intradermal and scarification routes, respectively, displayed a significantly higher number of lesions at 12 dpi than the group inoculated subcutaneously. However, the lesions following scarification spread from the inoculation site with no ORFV-associated lesions found in other areas of the mucous membrane or skin. Finally, following intradermal inoculation, ORFV-associated lesions developed homogeneously in all infected animals, with lesions progressing from the point of inoculation in different areas of the skin of the lips, yet also spreading to the interior of the mouth, gums, palate and tongue, as occurs in natural infections. Thus, it was concluded that for studies investigating the efficacy of new approaches to treatment and vaccination for improved welfare of affected animals and control of ORFV transmission, the most appropriate route for experimental ORFV infection is intradermal inoculation.

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来源期刊
Small Ruminant Research
Small Ruminant Research 农林科学-奶制品与动物科学
CiteScore
3.10
自引率
11.10%
发文量
210
审稿时长
12.5 weeks
期刊介绍: Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.
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