热休克肿瘤细胞产生的外泌体促进骨髓树突状细胞的体外成熟

IF 1.2 4区 医学 Q4 ALLERGY
Neda Heidari, Hajar Abbasi-Kenarsari, Bahare Niknam, Ali Asadirad, Davar Amani, Zahra Mirsanei, Seyed Mahmood Hashemi
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引用次数: 0

摘要

树突状细胞(DC)是利用 MHC II/I 分子处理和传递抗原的专业抗原递呈细胞,可以通过多种方式增强其功能。 从热休克肿瘤细胞(HS-TEXs)中提取的外泌体含有大量热休克蛋白(HSPs)。HSPs作为伴侣蛋白,可诱导直流电的成熟。本研究旨在探讨HS-TEXs是否能促进DC成熟。为了生成DC,骨髓衍生细胞经白细胞介素-4和GM-CSF处理。从热处理的CT-26细胞中分离出外泌体。用 Western 印迹法检测外泌体中 HSP 的表达水平,观察到该蛋白的表达增加。然后,将 HS-TEXs 与 iDCs 共同培养以测定 DC 的成熟度,再将 DCs 与淋巴细胞共同培养以测定 DC 的活性。我们的结果表明,经 HS-TEXs 处理的 DC 表达了高水平的参与 DC 成熟和功能的分子,包括 MHCII、CD40、CD83 和 CD86。HS-TEXs 可使 DCs 表型和功能成熟。此外,流式细胞计数结果显示,iDC / Tex + HSP 组淋巴细胞的增殖反应更强。HS-TEXs 可作为一种改善 DC 成熟和活化的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exosomes Derived from Heat-shocked Tumor Cells Promote In vitro Maturation of Bone Marrow-derived Dendritic Cells.

Dendritic cells (DCs), professional antigen-presenting cells that process and deliver antigens using MHC II/I molecules, can be enhanced in numerous ways.  Exosomes derived from heat-shocked tumor cells (HS-TEXs) contain high amounts of heat-shock proteins (HSPs). HSPs, as chaperons, can induce DC maturation. This study aimed to investigate whether HS-TEXs can promote DC maturation. To generate DC, bone marrow-derived cells were treated with Interleukin-4 and GM-CSF. Exosomes were isolated from heat-treated CT-26 cells. The expression level of HSP in exosomes was checked by western blot and the increase in the expression of this protein was observed. Then, HS-TEXs were co-cultured with iDCs to determine DC maturity, and then DCs were co-cultured with lymphocytes to determine DC activity. Our results showed that  DCs treated with HS-TEXs express high levels of molecules involved in DC maturation and function including MHCII, CD40, CD83, and CD86. HS-TEXs caused phenotypic and functional maturation of DCs. In addition, flow cytometric results reflected a higher proliferative response of lymphocytes in the iDC / Tex + HSP group. HS-TEXs could be used as a strategy to improve DC maturation and activation.

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来源期刊
CiteScore
2.60
自引率
6.70%
发文量
64
审稿时长
>12 weeks
期刊介绍: The Iranian Journal of Allergy, Asthma and Immunology (IJAAI), an international peer-reviewed scientific and research journal, seeks to publish original papers, selected review articles, case-based reviews, and other articles of special interest related to the fields of asthma, allergy and immunology. The journal is an official publication of the Iranian Society of Asthma and Allergy (ISAA), which is supported by the Immunology, Asthma and Allergy Research Institute (IAARI) and published by Tehran University of Medical Sciences (TUMS). The journal seeks to provide its readers with the highest quality materials published through a process of careful peer reviews and editorial comments. All papers are published in English.
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