毛细管区带电泳法定量检测治疗肽醋酸格拉替雷

Monatshefte für Chemie / Chemical Monthly Pub Date : 2024-03-14 DOI:10.1007/s00706-024-03190-8

{"title":"毛细管区带电泳法定量检测治疗肽醋酸格拉替雷","authors":"","doi":"10.1007/s00706-024-03190-8","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p>This study presents the development and validation of a novel capillary zone electrophoresis method for the precise determination of glatiramer acetate and its amino acid constituents. A 120 mmol dm<sup>−3</sup> phosphoric acid solution adjusted to pH 1.9 with Tris, supplemented with 20 mmol dm<sup>−3</sup> triethylamine to achieve a final of pH 2.1, resulted in a repeatable analysis of glatiramer acetate. The method demonstrated a limit of detection and quantification of 39.2 µg cm<sup>−3</sup> and 130.7 µg cm<sup>−3</sup>, respectively. This method allows for the rapid control of glatiramer acetate-based pharmaceuticals and distinguishes glatiramer acetate from the amino acids used in its synthesis.</p> <span> <h3>Graphical abstract</h3> <p> <span> <span> <img alt=\"\" src=\"https://static-content.springer.com/image/MediaObjects/706_2024_3190_Figa_HTML.png\"/> </span> </span></p> </span>","PeriodicalId":19011,"journal":{"name":"Monatshefte für Chemie / Chemical Monthly","volume":"2018 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Capillary zone electrophoresis method for quantification of therapeutic peptide glatiramer acetate\",\"authors\":\"\",\"doi\":\"10.1007/s00706-024-03190-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3>Abstract</h3> <p>This study presents the development and validation of a novel capillary zone electrophoresis method for the precise determination of glatiramer acetate and its amino acid constituents. A 120 mmol dm<sup>−3</sup> phosphoric acid solution adjusted to pH 1.9 with Tris, supplemented with 20 mmol dm<sup>−3</sup> triethylamine to achieve a final of pH 2.1, resulted in a repeatable analysis of glatiramer acetate. The method demonstrated a limit of detection and quantification of 39.2 µg cm<sup>−3</sup> and 130.7 µg cm<sup>−3</sup>, respectively. This method allows for the rapid control of glatiramer acetate-based pharmaceuticals and distinguishes glatiramer acetate from the amino acids used in its synthesis.</p> <span> <h3>Graphical abstract</h3> <p> <span> <span> <img alt=\\\"\\\" src=\\\"https://static-content.springer.com/image/MediaObjects/706_2024_3190_Figa_HTML.png\\\"/> </span> </span></p> </span>\",\"PeriodicalId\":19011,\"journal\":{\"name\":\"Monatshefte für Chemie / Chemical Monthly\",\"volume\":\"2018 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Monatshefte für Chemie / Chemical Monthly\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/s00706-024-03190-8\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Monatshefte für Chemie / Chemical Monthly","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s00706-024-03190-8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

摘要本研究开发并验证了一种新型毛细管区带电泳方法，用于精确测定醋酸格拉替雷及其氨基酸成分。用三羟甲基氨基甲烷将 120 mmol dm-3 磷酸溶液调节至 pH 1.9，再加入 20 mmol dm-3 三乙胺使最终 pH 值达到 2.1，从而实现了对醋酸格拉替雷的重复性分析。该方法的检出限和定量限分别为 39.2 µg cm-3 和 130.7 µg cm-3。该方法可快速控制醋酸格拉替雷药物，并将醋酸格拉替雷与其合成过程中使用的氨基酸区分开来。图表摘要

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Capillary zone electrophoresis method for quantification of therapeutic peptide glatiramer acetate

Abstract

This study presents the development and validation of a novel capillary zone electrophoresis method for the precise determination of glatiramer acetate and its amino acid constituents. A 120 mmol dm⁻³ phosphoric acid solution adjusted to pH 1.9 with Tris, supplemented with 20 mmol dm⁻³ triethylamine to achieve a final of pH 2.1, resulted in a repeatable analysis of glatiramer acetate. The method demonstrated a limit of detection and quantification of 39.2 µg cm⁻³ and 130.7 µg cm⁻³, respectively. This method allows for the rapid control of glatiramer acetate-based pharmaceuticals and distinguishes glatiramer acetate from the amino acids used in its synthesis.

Graphical abstract

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Monatshefte für Chemie / Chemical Monthly

自引率

0.00%

发文量