重新利用脱细胞肺来生成血管化脂肪。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3762-3_18

Lindsey K Huff, Zihan Ling, Megan K DeBari, Xi Ren, Rosalyn D Abbott

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引用次数: 0

摘要

解决皮下脂肪组织严重缺损的传统疗法仍然是外科医生面临的重建挑战，这主要是由于缺乏移植前血管化。脂肪组织依赖于密集的微血管网络来输送营养、氧气、非脂肪组织衍生的生长因子、细胞因子和激素，并将脂肪组织衍生的内分泌信号输送到其他器官系统。本章通过将脱细胞肺基质与患者特异性脂肪来源干细胞和内皮细胞的分步播种相结合，来开发大容量、可灌注和预血管化的脂肪移植物，从而解决这些血管化问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Repurposing Decellularized Lung to Generate Vascularized Fat.

Conventional therapies to address critically sized defects in subcutaneous adipose tissue remain a reconstructive challenge for surgeons, largely due to the lack of graft pre-vascularization. Adipose tissue relies on a dense microvasculature network to deliver nutrients, oxygen, nonadipose tissue-derived growth factors, cytokines, and hormones, as well as transporting adipose tissue-derived endocrine signals to other organ systems. This chapter addresses these vascularization issues by combining decellularized lung matrices with a step-wise seeding of patient-specific adipose-derived stem cells and endothelial cells to develop large-volume, perfusable, and pre-vascularized adipose grafts.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.