通过循环静水压、模拟微重力和循环拉伸应变对脂肪基质/干细胞进行机械刺激，以实现肌肉骨骼系统的功能性组织工程学。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3762-3_25

Rachel C Nordberg, Josie C Bodle, Elizabeth G Loboa

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引用次数: 0

摘要

人体脂肪基质/干细胞（hASC）组织工程疗法必须具备适当的机械特性，才能恢复肌肉骨骼系统承重组织的功能。为了阐明 hASC 对机械刺激的反应并开发出具有机械稳健性的组织工程构建物，最近的研究利用了多种机械加载范例，包括循环拉伸应变、循环静水压和模拟微重力下的机械卸载。本章将介绍对 hASC 施加这些机械刺激的方法，以引导分化，从而实现肌肉骨骼系统的功能性组织工程。

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Mechanical Stimulation of Adipose-Derived Stromal/Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain.

It is critical that human adipose-derived stromal/stem cell (hASC) tissue engineering therapies possess appropriate mechanical properties in order to restore the function of the load-bearing tissues of the musculoskeletal system. In an effort to elucidate hASC response to mechanical stimulation and develop mechanically robust tissue-engineered constructs, recent research has utilized a variety of mechanical loading paradigms, including cyclic tensile strain, cyclic hydrostatic pressure, and mechanical unloading in simulated microgravity. This chapter will describe the methods for applying these mechanical stimuli to hASC to direct differentiation for functional tissue engineering of the musculoskeletal system.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.