使用基于反式-4-羟基-L-脯氨醇的亚磷酰胺试剂合成含硫醇的 DNA-寡核苷酸

Q4 Chemistry

Proceedings of the National Academy of Sciences of Belarus, Chemical Series Pub Date : 2024-03-04 DOI:10.29235/1561-8331-2024-60-1-36-44

Y. Lamekina, T. Seviarynchyk, E. Ulashchik, T. P. Akhlamionok, P. Y. Baryshchyk, B. Ranishenka, V. V. Shmanai

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引用次数: 0

摘要

在这项工作中，合成了一种用于制备硫醇修饰寡核苷酸的新型亚磷酰胺试剂。硫醇特异性试剂用于证明硫醇基团的反应性。修饰后的共轭物经凝胶过滤纯化，并用 HPLC-MS 进行表征。

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Synthesis of thiol-containing DNA-oligonucleotides using a phosphoramidite reagent based on trans -4-hydroxy-L-prolinol

In this work, a new phosphoramidite reagent for the preparation of thiol-modified oligonucleotides was synthesized. Thiol-specific reagents have been used to demonstrate the reactivity of thiol groups. After modification, conjugates were purified via gel-filtration and characterized with HPLC-MS.

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来源期刊

Proceedings of the National Academy of Sciences of Belarus, Chemical Series Chemistry-Chemistry (miscellaneous)

CiteScore

0.30

自引率

0.00%

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