从绒毛取样中生成滋养层细胞器质

Organoids Pub Date : 2024-03-05 DOI:10.3390/organoids3010005
B. V. van Rijn, Diane Van Opstal, Nicole van Koetsveld, Maarten Knapen, Joost Gribnau, Olivier Schäffers
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引用次数: 0

摘要

由于在整个妊娠过程中获取和维持胎盘组织存在固有的困难,因此研究人类胎盘的发育和功能面临着巨大的挑战。在这里,我们提供了一种从妊娠期获得的绒毛中生成滋养层细胞器质的详细方案。我们的方法能有效地从绒毛取样中生成滋养层细胞器质,无需预选绒毛,并能控制蜕膜腺器质的污染。生成的滋养细胞器质会自发形成合胞滋养细胞,并开始分泌 hCG 激素和其他胎盘特异性因子。我们的方法有助于从各种遗传背景(包括三体和基因突变)中生成滋养细胞器质,并可与产前诊断程序保持一致。该方案最长需要 14 天,具有细胞培养专业知识的用户均可操作。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Generation of Trophoblast Organoids from Chorionic Villus Sampling
Studying human placental development and function presents significant challenges due to the inherent difficulties in obtaining and maintaining placental tissue throughout the course of an ongoing pregnancy. Here, we provide a detailed protocol for generating trophoblast organoids from chorionic villi obtained during ongoing pregnancy. Our method results in efficient generation of trophoblast organoids from chorionic villus sampling, does not require preselection of chorionic villi, and controls contamination of decidual gland organoids. The resulting trophoblast organoids spontaneously form syncytiotrophoblasts that start secreting hCG hormone amongst other placenta-specific factors. Our approach facilitates the generation of trophoblast organoids from a variety of genetic backgrounds, including trisomies and gene mutations, and can be aligned with prenatal diagnostic routines. The protocol requires up to 14 days and can be carried out by users with expertise in cell culture.
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