R. O. Kulibaba, Yu. V. Liashenko, M. I. Sakhatskyi
{"title":"乌克兰荷斯坦牛群中 CSN2 和 TNF-α 基因的多态性","authors":"R. O. Kulibaba, Yu. V. Liashenko, M. I. Sakhatskyi","doi":"10.3103/s0095452724010055","DOIUrl":null,"url":null,"abstract":"<p>Peculiarities of the genetic structure of the population of Holstein cattle bred in Ukraine were studied by beta-casein and tumor necrosis factor alpha loci. Using the methods of allele-specific PCR (AS-PCR) and PCR with restriction fragment length polymorphism (PCR-RFLP) analysis, polymorphism of the beta-casein gene (<i>CSN2</i>) by allelic variants A<sup>1</sup> and A<sup>2</sup> and of the tumor necrosis factor-alpha (<i>TNF</i>-α) by SacI-polymorphism in the promoter region of the gene (–824 A>G marker mutation) and RsaI-polymorphism in the fourth exon were analyzed. It was proven that both loci are polymorphic in the studied cattle population. For each of the polymorphic loci, the main genetic and population parameters of the Holstein cow population were established. For the <i>CSN2</i> locus, a significant predominance of the frequency of the A<sup>2</sup> allele over A<sup>1</sup> was established (0.78 vs. 0.22). For the SacI- and RsaI-polymorphisms of <i>TNF</i>-α, maximal (1.980 and 1.988) values of the locus polymorphism level (the number of effective alleles) were detected. For both mutations in the <i>TNF</i>-α gene, actual parity in the frequency values of the corresponding alleles was established (0.55 and 0.45 for the SacI-polymorphism; 0.54 and 0.46 for the RsaI-polymorphism). For the RsaI-polymorphism in the <i>TNF</i>-α fourth exon, a deviation from the state of genetic equilibrium according to Hardy–Weinberg was recorded in the studied population of animals, and a significant excess of heterozygous individuals was detected (25%). Based on the results of the analysis of milk productivity parameters of individuals with different <i>CSN2</i> locus genotypes, it was established that individuals with the A<sup>2</sup>A<sup>2</sup> genotype are characterized by higher values of standard milk production as compared with individuals with the A<sup>1</sup>A<sup>1</sup> genotype (<i>p</i> = 0.042). For the SacI- and RsaI-polymorphism of the <i>TNF</i>-α gene, there were no significant differences in the parameters of standard milk productivity for two lactations between individuals with different genotypes.</p>","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Polymorphism of CSN2 and TNF-α Genes in the Population of Holstein Cattle Bred in Ukraine\",\"authors\":\"R. O. Kulibaba, Yu. V. Liashenko, M. I. Sakhatskyi\",\"doi\":\"10.3103/s0095452724010055\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Peculiarities of the genetic structure of the population of Holstein cattle bred in Ukraine were studied by beta-casein and tumor necrosis factor alpha loci. Using the methods of allele-specific PCR (AS-PCR) and PCR with restriction fragment length polymorphism (PCR-RFLP) analysis, polymorphism of the beta-casein gene (<i>CSN2</i>) by allelic variants A<sup>1</sup> and A<sup>2</sup> and of the tumor necrosis factor-alpha (<i>TNF</i>-α) by SacI-polymorphism in the promoter region of the gene (–824 A>G marker mutation) and RsaI-polymorphism in the fourth exon were analyzed. It was proven that both loci are polymorphic in the studied cattle population. For each of the polymorphic loci, the main genetic and population parameters of the Holstein cow population were established. For the <i>CSN2</i> locus, a significant predominance of the frequency of the A<sup>2</sup> allele over A<sup>1</sup> was established (0.78 vs. 0.22). For the SacI- and RsaI-polymorphisms of <i>TNF</i>-α, maximal (1.980 and 1.988) values of the locus polymorphism level (the number of effective alleles) were detected. For both mutations in the <i>TNF</i>-α gene, actual parity in the frequency values of the corresponding alleles was established (0.55 and 0.45 for the SacI-polymorphism; 0.54 and 0.46 for the RsaI-polymorphism). For the RsaI-polymorphism in the <i>TNF</i>-α fourth exon, a deviation from the state of genetic equilibrium according to Hardy–Weinberg was recorded in the studied population of animals, and a significant excess of heterozygous individuals was detected (25%). Based on the results of the analysis of milk productivity parameters of individuals with different <i>CSN2</i> locus genotypes, it was established that individuals with the A<sup>2</sup>A<sup>2</sup> genotype are characterized by higher values of standard milk production as compared with individuals with the A<sup>1</sup>A<sup>1</sup> genotype (<i>p</i> = 0.042). For the SacI- and RsaI-polymorphism of the <i>TNF</i>-α gene, there were no significant differences in the parameters of standard milk productivity for two lactations between individuals with different genotypes.</p>\",\"PeriodicalId\":0,\"journal\":{\"name\":\"\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0,\"publicationDate\":\"2024-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3103/s0095452724010055\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3103/s0095452724010055","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Polymorphism of CSN2 and TNF-α Genes in the Population of Holstein Cattle Bred in Ukraine
Peculiarities of the genetic structure of the population of Holstein cattle bred in Ukraine were studied by beta-casein and tumor necrosis factor alpha loci. Using the methods of allele-specific PCR (AS-PCR) and PCR with restriction fragment length polymorphism (PCR-RFLP) analysis, polymorphism of the beta-casein gene (CSN2) by allelic variants A1 and A2 and of the tumor necrosis factor-alpha (TNF-α) by SacI-polymorphism in the promoter region of the gene (–824 A>G marker mutation) and RsaI-polymorphism in the fourth exon were analyzed. It was proven that both loci are polymorphic in the studied cattle population. For each of the polymorphic loci, the main genetic and population parameters of the Holstein cow population were established. For the CSN2 locus, a significant predominance of the frequency of the A2 allele over A1 was established (0.78 vs. 0.22). For the SacI- and RsaI-polymorphisms of TNF-α, maximal (1.980 and 1.988) values of the locus polymorphism level (the number of effective alleles) were detected. For both mutations in the TNF-α gene, actual parity in the frequency values of the corresponding alleles was established (0.55 and 0.45 for the SacI-polymorphism; 0.54 and 0.46 for the RsaI-polymorphism). For the RsaI-polymorphism in the TNF-α fourth exon, a deviation from the state of genetic equilibrium according to Hardy–Weinberg was recorded in the studied population of animals, and a significant excess of heterozygous individuals was detected (25%). Based on the results of the analysis of milk productivity parameters of individuals with different CSN2 locus genotypes, it was established that individuals with the A2A2 genotype are characterized by higher values of standard milk production as compared with individuals with the A1A1 genotype (p = 0.042). For the SacI- and RsaI-polymorphism of the TNF-α gene, there were no significant differences in the parameters of standard milk productivity for two lactations between individuals with different genotypes.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
