Yo-han Choi, Woo-Joong Kim, Dongwoo Lee, Bum-Joon Jung, Eui-Cheol Shin, Wonhee Lee
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引用次数: 0
摘要
在人类 CD8+ T 细胞中,衰老细胞以表达 CD57 为标志。衰老的 CD57+CD8+ T 细胞的频率与衰老和年龄相关疾病密切相关,可以通过多色流式细胞术测量。然而,多色流式细胞术在可及性方面存在挑战,并且需要大量的资源分配。因此,开发一种快速、直接的方法来量化 CD57+CD8+ T 细胞仍是一项关键挑战。本研究介绍了一种微流控装置,该装置由一个 PDMS 微流控通道和一个用于固定抗 CD8 抗体的预修饰玻璃基底组成。这种设计可使血液样本流过,从而选择性地捕获 CD8+ T 细胞,同时最大限度地减少所需的血液样本量。这项技术可通过荧光图像分析对捕获的 CD8+ T 细胞中的 CD57+ 细胞进行准确可靠的量化。该设备能够轻松量化衰老的 CD57+CD8+ T 细胞,预计将极大地促进免疫学研究和临床应用。
Microfluidic Capture Device for Simple, Cell Surface Marker-Based Quantification of Senescent CD8+ T Cells
Among human CD8+ T cells, senescent cells are marked by the expression of CD57. The frequency of senescent CD57+CD8+ T cells is significantly correlated with aging and age-associated disorders, and it can be measured by multi-color flow cytometry. However, multi-color flow cytometry presents challenges in terms of accessibility and requires significant resource allocation. Therefore, developing a rapid and straightforward method for quantifying CD57+CD8+ T cells remains a key challenge. This study introduces a microfluidic device composed of a PDMS microfluidic channel with a pre-modified glass substrate for anti-CD8 antibody immobilization. This design allows blood samples to flow through, enabling the selective capture of CD8+ T cells while minimizing the required blood sample volume. This technology enables accurate and reliable quantification of CD57+ cells among captured CD8+ T cells through fluorescence image analysis. The ability of the device to easily quantify senescent CD57+CD8+ T cells is anticipated to contribute significantly to both immunological research and clinical applications.
期刊介绍:
BioChip Journal publishes original research and reviews in all areas of the biochip technology in the following disciplines, including protein chip, DNA chip, cell chip, lab-on-a-chip, bio-MEMS, biosensor, micro/nano mechanics, microfluidics, high-throughput screening technology, medical science, genomics, proteomics, bioinformatics, medical diagnostics, environmental monitoring and micro/nanotechnology. The Journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.