利用独特的纳米粒子比色法在单检测线侧流免疫分析仪中同时进行三重检测

IF 5.5 3区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Youngkwang Moon, Hui Hun Cho, Hyeokgyun Moon, Hyewon Song, Jae Chul Ro, Jung Heon Lee, Jinkee Lee
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引用次数: 0

摘要

侧流免疫分析法(LFIA)已成为快速检测生物分子的常用方法,同时也出现了对多重检测能力的需求。我们开发了一种新型 LFIA 设备,它能在一条检测线上同时检测三种不同的抗原,每种抗原都有独特的颜色。金纳米颗粒(AuNPs;红色)、金纳米棒(AuNRs;蓝色)和银纳米颗粒(AgNPs;黄色)被设计成在 LFIA 装置内同时流动,并与检测线上的α-胎儿蛋白(AFP)、神经元特异性烯醇化酶(NSE)和癌胚抗原(CEA)发生特异性反应。该装置能有效地单独和同时检测分析物,检测限(LOD)为 50 纳克/毫升。鉴于该多重检测 LFIA 设备反应迅速、易于使用且价格低廉,它在食品质量管理、牲畜诊断以及健康和环境监测等广泛应用中展现出巨大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Simultaneous Triplex Detection in a Single-Test-Line Lateral Flow Immunoassay Utilizing Distinct Nanoparticle Colorimetry

Simultaneous Triplex Detection in a Single-Test-Line Lateral Flow Immunoassay Utilizing Distinct Nanoparticle Colorimetry

Lateral flow immunoassay (LFIA) has become a popular method for the rapid detection of biological molecules, with an emerging need for multiplex detection capabilities. A novel LFIA device capable of simultaneously detecting three different antigens on a single test line was developed, with each antigen identifiable by a unique color. Gold nanoparticles (AuNPs; red), gold nanorods (AuNRs; blue), and silver nanoparticles (AgNPs; yellow) were engineered to flow concurrently within the LFIA device and specifically react with α-fetoprotein (AFP), neuron-specific enolase (NSE), and carcinoembryonic antigen (CEA) on the test line. The device was effective for both individual and simultaneous detection of the analytes, with a limit of detection (LOD) of 50 ng/mL. Given its rapid response, ease of use, and affordability, this multiplex detection LFIA device shows great potential for a wide range of applications, including food quality management, livestock diagnosis, and health and environmental monitoring.

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来源期刊
BioChip Journal
BioChip Journal 生物-生化研究方法
CiteScore
7.70
自引率
16.30%
发文量
47
审稿时长
6-12 weeks
期刊介绍: BioChip Journal publishes original research and reviews in all areas of the biochip technology in the following disciplines, including protein chip, DNA chip, cell chip, lab-on-a-chip, bio-MEMS, biosensor, micro/nano mechanics, microfluidics, high-throughput screening technology, medical science, genomics, proteomics, bioinformatics, medical diagnostics, environmental monitoring and micro/nanotechnology. The Journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.
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