在电纺纳米纤维上同时涂覆生物活性分子,促进体外干细胞成骨。

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Mehrdad Zahiri-Toosi, Seyed Jalal Zargar, Ehsan Seyedjafari, Mostafa Saberian, Marziehsadat Ahmadi
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引用次数: 0

摘要

目的:间充质干细胞(MSCs)具有分泌和再生生物活性分子的能力,因此被广泛认为是一种具有治疗应用前景的细胞类型。要实现有效的骨愈合,选择一种能支持、诱导和恢复生物功能的支架至关重要。评估支架应包括评估间充质干细胞的存活、增殖和分化。本研究的主要目的是制备适合骨组织工程应用的复合纳米纤维支架:在这项实验研究中,使用聚左旋乳酸(PLLA)聚合物制作了纳米纤维支架。结果表明,胶原-L-乳酸(PLLA)和羟基磷灰石(HA)纳米颗粒的结合对 PLLA 纤维的表面进行了修饰:结果:研究结果表明,胶原和纳米羟基磷灰石修饰的电纺聚乳酸支架对间叶干细胞的附着、生长和成骨分化有积极影响:结论:在纳米纤维支架上涂覆胶原蛋白和纳米羟基磷灰石能显著提高间充质干细胞在电纺聚乳酸支架上的成骨分化能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In Vitro.

Objective: Mesenchymal stem cells (MSCs) are widely recognized as a promising cell type for therapeutic applications due to their ability to secrete and regenerate bioactive molecules. For effective bone healing, it is crucial to select a scaffold that can support, induce, and restore biological function. Evaluating the scaffold should involve assessing MSC survival, proliferation, and differentiation. The principal aim of this investigation was to formulate composite nanofibrous scaffolds apt for applications in bone tissue engineering.

Materials and methods: In this experimental study, nanofibrous scaffolds were fabricated using Poly-L-lactic acid (PLLA) polymer. The PLLA fibers' surface was modified by integrating collagen and hydroxyapatite (HA) nanoparticles.

Results: The findings demonstrated that the collagen- and nanohydroxyapatite-modified electrospun PLLA scaffold positively influenced the attachment, growth, and osteogenic differentiation of MSCs.

Conclusion: Coating the nanofiber scaffold with collagen and nanoparticle HA significantly enhanced the osteogenic differentiation of MSCs on electrospun PLLA scaffolds.

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来源期刊
Cell Journal
Cell Journal CELL BIOLOGY-
CiteScore
3.40
自引率
5.00%
发文量
0
审稿时长
12 months
期刊介绍: The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.
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