{"title":"奥沙利铂和普尼卡星对结肠癌细胞 Caco-2 死亡的协同作用。","authors":"Hadeil Muhanna Alsufiani","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>The objectives of the study are to investigate the synergistic effect of oxaliplatin (oxa) and punicalagin (pun) on the death of colon cancer cells (Caco-2) by apoptosis and autophagy.</p><p><strong>Methods: </strong>The effects of the combined treatments (5 μM oxa + 50 μM pun, 5 μM oxa + 75 μM pun, 20 μM oxa + 50 μM pun, and 5 μM oxa + 75 μM pun) were compared with untreated Caco2 cells (control) or cells treated with oxa alone. Apoptosis was detected using an Annex in V FITC flow cytometry assay and poly (ADP-ribose) polymerase cleavage by western blotting. Light chain 3 was detected by western blotting as an autophagy marker.</p><p><strong>Results: </strong>The combined treatments significantly increased the number of apoptotic cells in comparison to untreated cells or cells treated with oxa alone. By contrast, the combined treatments had no significant effect on autophagy.</p><p><strong>Conclusion: </strong>The combined treatment significantly promoted cell death through apoptosis while maintaining a basal level of autophagy.</p>","PeriodicalId":47093,"journal":{"name":"International Journal of Health Sciences-IJHS","volume":"18 2","pages":"33-37"},"PeriodicalIF":2.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10915914/pdf/","citationCount":"0","resultStr":"{\"title\":\"The synergistic effect of oxaliplatin and punicalagin on colon cancer cells Caco-2 death.\",\"authors\":\"Hadeil Muhanna Alsufiani\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>The objectives of the study are to investigate the synergistic effect of oxaliplatin (oxa) and punicalagin (pun) on the death of colon cancer cells (Caco-2) by apoptosis and autophagy.</p><p><strong>Methods: </strong>The effects of the combined treatments (5 μM oxa + 50 μM pun, 5 μM oxa + 75 μM pun, 20 μM oxa + 50 μM pun, and 5 μM oxa + 75 μM pun) were compared with untreated Caco2 cells (control) or cells treated with oxa alone. Apoptosis was detected using an Annex in V FITC flow cytometry assay and poly (ADP-ribose) polymerase cleavage by western blotting. Light chain 3 was detected by western blotting as an autophagy marker.</p><p><strong>Results: </strong>The combined treatments significantly increased the number of apoptotic cells in comparison to untreated cells or cells treated with oxa alone. By contrast, the combined treatments had no significant effect on autophagy.</p><p><strong>Conclusion: </strong>The combined treatment significantly promoted cell death through apoptosis while maintaining a basal level of autophagy.</p>\",\"PeriodicalId\":47093,\"journal\":{\"name\":\"International Journal of Health Sciences-IJHS\",\"volume\":\"18 2\",\"pages\":\"33-37\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10915914/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Health Sciences-IJHS\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Health Sciences-IJHS","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0
摘要
研究目的本研究旨在探讨奥沙利铂（oxa）和潘生丁（punicalagin）通过凋亡和自噬对结肠癌细胞（Caco-2）死亡的协同作用：与未处理的 Caco2 细胞（对照组）或单用奥沙利铂处理的细胞相比，比较了联合处理（5 μM 奥沙利铂 + 50 μM pun、5 μM 奥沙利铂 + 75 μM pun、20 μM 奥沙利铂 + 50 μM pun 和 5 μM 奥沙利铂 + 75 μM pun）的效果。细胞凋亡采用 Annex in V FITC 流式细胞术检测，多聚（ADP-核糖）聚合酶裂解采用蛋白印迹法检测。轻链 3 作为自噬标记物通过 Western 印迹法进行检测：结果：与未处理的细胞或单用 oxa 处理的细胞相比，联合处理可明显增加凋亡细胞的数量。相比之下，联合处理对自噬没有明显影响：结论：联合疗法在维持自噬基础水平的同时，能明显促进细胞凋亡。
The synergistic effect of oxaliplatin and punicalagin on colon cancer cells Caco-2 death.
Objectives: The objectives of the study are to investigate the synergistic effect of oxaliplatin (oxa) and punicalagin (pun) on the death of colon cancer cells (Caco-2) by apoptosis and autophagy.
Methods: The effects of the combined treatments (5 μM oxa + 50 μM pun, 5 μM oxa + 75 μM pun, 20 μM oxa + 50 μM pun, and 5 μM oxa + 75 μM pun) were compared with untreated Caco2 cells (control) or cells treated with oxa alone. Apoptosis was detected using an Annex in V FITC flow cytometry assay and poly (ADP-ribose) polymerase cleavage by western blotting. Light chain 3 was detected by western blotting as an autophagy marker.
Results: The combined treatments significantly increased the number of apoptotic cells in comparison to untreated cells or cells treated with oxa alone. By contrast, the combined treatments had no significant effect on autophagy.
Conclusion: The combined treatment significantly promoted cell death through apoptosis while maintaining a basal level of autophagy.
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